外壳蛋白序列显示，从天竺葵（天竺葵属）分离出的黄瓜花叶病毒属于亚组II。

Coat protein sequence shows that Cucumber mosaic virus isolate from geraniums (Pelargonium spp.) belongs to subgroup II.

作者信息

Verma Neeraj, Mahalingam B K, Ram Raja, Zaidi A A

机构信息

Plant Virus Lab, Floriculture Division, Institute of Himalayan Bioresource Technology, Palampur, India.

出版信息

J Biosci. 2006 Mar;31(1):47-54. doi: 10.1007/BF02705234.

DOI:10.1007/BF02705234

PMID:16595874

Abstract

A viral disease was identified on geraniums (Pelargonium spp.) grown in a greenhouse at the Institute of Himalayan Bioresource Technology (IHBT), Palampur, exhibiting mild mottling and stunting. The causal virus (Cucumber mosaic virus, CMV) was identified and characterized on the basis of host range, aphid transmission, enzyme linked immunosorbent assay (ELISA), DNA-RNA hybridization and reverse transcription polymerase chain reaction (RT-PCR). A complete coat protein (CP) gene was amplified using degenerate primers and sequenced. The CP gene showed nucleotide and amino acid homology up to 97%-98% and 96%-99%, respectively with the sequences of CMV subgroup II. The CP gene also showed homologies of 75%-97% in nucleotide and 77%-96% in amino acid with the CMV Indian isolates infecting various crops. On the basis of sequence homology, it was concluded that CMV-infecting geraniums in India belong to subgroup II.

摘要

在位于帕拉姆布尔的喜马拉雅生物资源技术研究所（IHBT）的温室中种植的天竺葵（天竺葵属）上发现了一种病毒性疾病，其表现为轻度斑驳和发育迟缓。基于寄主范围、蚜虫传播、酶联免疫吸附测定（ELISA）、DNA - RNA杂交以及逆转录聚合酶链反应（RT-PCR）对致病病毒（黄瓜花叶病毒，CMV）进行了鉴定和特征分析。使用简并引物扩增并测序了完整的外壳蛋白（CP）基因。该CP基因与CMV亚组II的序列在核苷酸和氨基酸水平上的同源性分别高达97% - 98%和96% - 99%。该CP基因与感染各种作物的CMV印度分离株在核苷酸水平上的同源性为75% - 97%，在氨基酸水平上的同源性为77% - 96%。基于序列同源性，得出结论：在印度感染天竺葵的CMV属于亚组II。