Hu C C, Aboul-Ata A E, Naidu R A, Ghabrial S A
Department of Plant Pathology, University of Kentucky, Lexington 40546, USA.
J Gen Virol. 1997 Apr;78 ( Pt 4):929-39. doi: 10.1099/0022-1317-78-4-929.
Strains of peanut stunt cucumovirus (PSV) were classified into two distinct subgroups, I and II, based on Western and Northern blot analyses using antisera and cloned cDNA probes to strains PSV-ER and PSV-W. These results were corroborated by nucleotide sequence analyses of full-length cDNA clones of RNA3 from representative strains of the two subgroups. Whereas the percentage nucleotide sequence identity between PSV-ER (or PSV-J) and PSV-W RNA3s was determined to be 80%, the corresponding value between strains ER and J was 91%, confirming that strains ER and J belong to the same subgroup (subgroup I) whereas strain W belongs to a separate subgroup (subgroup II). PSV-W and PSV-ER RNA3s are 2173 and 2188 nucleotides long, respectively. Each is dicistronic, encoding a putative movement protein (3a protein) and a coat protein (CP). The intercistronic and 5' untranslated region (UTR) sequences of PSV strains, unlike those of cucumber mosaic cucumovirus (CMV) strains, are highly conserved and thus not useful for distinguishing the two subgroups. However, the 3' UTR sequences of PSV strains, like those of CMV strains, can discriminate between the two subgroups since strains within the same subgroup are 95% identical in their 3' UTRs whereas those in different subgroups are only 74-78% identical. PSV-W and PSV-ER RNA4s were determined to be 994 and 1006 nucleotides long, respectively. PSV 3a and CP genes have higher percentage nucleotide sequence identities to those of tomato aspermy cucumovirus than to those of CMV.
花生矮化黄瓜花叶病毒（PSV）毒株基于使用针对PSV-ER和PSV-W毒株的抗血清及克隆cDNA探针进行的蛋白质免疫印迹分析和Northern印迹分析，被分为两个不同的亚组，即亚组I和亚组II。这一结果得到了来自这两个亚组代表性毒株的RNA3全长cDNA克隆的核苷酸序列分析的证实。虽然PSV-ER（或PSV-J）与PSV-W的RNA3之间的核苷酸序列同一性百分比被确定为80%，但ER毒株和J毒株之间的相应值为91%，这证实ER毒株和J毒株属于同一亚组（亚组I），而W毒株属于另一个亚组（亚组II）。PSV-W和PSV-ER的RNA3分别长2173和2188个核苷酸。它们均为双顺反子，编码一个假定的运动蛋白（3a蛋白）和一个外壳蛋白（CP）。与黄瓜花叶黄瓜花叶病毒（CMV）毒株不同，PSV毒株的顺反子间和5'非翻译区（UTR）序列高度保守，因此对于区分这两个亚组并无用处。然而，与CMV毒株一样，PSV毒株的3'UTR序列能够区分这两个亚组，因为同一亚组内的毒株其3'UTR的同一性为95%，而不同亚组的毒株之间仅为74 - 78%相同。PSV-W和PSV-ER的RNA4分别被确定为长994和1006个核苷酸。与CMV相比，PSV的3a和CP基因与番茄不孕黄瓜花叶病毒具有更高的核苷酸序列同一性百分比。
