Crespo M O Punín, Martínez M Vilasoa, Hernández J López, Lage Yusty M A
Department of Analytical Chemistry, Nutrition and Bromatology, Faculty of Pharmacy, University of Santiago de Compostela, 15782 Santiago de Compostela, Spain.
J Chromatogr A. 2006 May 26;1116(1-2):189-92. doi: 10.1016/j.chroma.2006.03.068. Epub 2006 Apr 4.
A HPLC method was developed for the determination of glucosamine in crab shells. The amount of glucosamine residue can be used to estimate the amount of chitin in analyzed material. Crab samples were hydrolysed with 6M HCl at 100 degrees C for 13 h and the released glucosamine was determined by HPLC using refractive index (RI) detection. Separations were performed using an ODS-2 column with an isocratic mobile phase consisting of 10% methanol, 90% hexanesulfonate, pH 2.1. Glucosamine identification was carried out not only on basis of retention time, but also with respect to mass spectra, which were acquired by HPLC-MS.
建立了一种用于测定蟹壳中氨基葡萄糖的高效液相色谱法。氨基葡萄糖残留量可用于估算分析材料中几丁质的含量。蟹类样品在100℃下用6M盐酸水解13小时，释放出的氨基葡萄糖通过高效液相色谱法采用示差折光检测法进行测定。使用ODS-2柱进行分离，等度流动相由10%甲醇、90%己烷磺酸盐组成，pH值为2.1。氨基葡萄糖的鉴定不仅基于保留时间，还基于通过高效液相色谱-质谱获得的质谱图。
