通过二维凝胶电泳分析一种新型遗传性肝炎和肝癌突变体（LEC大鼠）肝脏中的多肽：鉴定P29/6.8为碳酸酐酶III和磷酸丙糖异构酶。

Analyses of polypeptides in the liver of a novel mutant (LEC rats) to hereditary hepatitis and hepatoma by two-dimensional gel electrophoresis: identification of P29/6.8 as carbonic anhydrase III and triosephosphate isomerase.

Nagase T, Sugiyama T, Kawata S, Tarui S, Deutsch H F, Taniguchi N

Department of Biochemistry, Osaka University Medical School, Japan.

Comp Biochem Physiol B. 1991;99(1):193-201. doi: 10.1016/0305-0491(91)90029-d.

Abstract

Total cellular proteins from the livers of 4-, 16- and 52-week-old hepatitis- and hepatoma-predisposed Long-Evans Cinnamon (LEC) rats were compared to those from the livers of the corresponding control rats [Long-Evans Agouti (LEA) rats] by two-dimensional gel electrophoresis. 2. A polypeptide, p50/7.2 (molecular weight x 10(-3)/isoelectric point) was only found in the LEC rats, and the p43/6.4 component was greater and the p51/6.8 component was less in the LEC rats than in the LEA rats during aging. 3. A polypeptide, p29/6.8, was dramatically greater in 4-week-old LEC rats than in 4-week-old LEA rats. 4. By sequencing and Western blotting analysis, the marked differences in the level of the p29/6.8 component were found to be due to carbonic anhydrase III.

摘要

通过二维凝胶电泳，将4周龄、16周龄和52周龄的肝炎和肝癌易感长Evans肉桂色（LEC）大鼠肝脏中的总细胞蛋白与相应对照大鼠（长Evans刺鼠色，LEA大鼠）肝脏中的总细胞蛋白进行比较。2. 一种名为p50/7.2（分子量×10⁻³/等电点）的多肽仅在LEC大鼠中被发现，并且在衰老过程中，LEC大鼠中的p43/6.4组分比LEA大鼠中的更大，而p51/6.8组分比LEA大鼠中的更少。3. 一种名为p29/6.8的多肽在4周龄的LEC大鼠中比在4周龄的LEA大鼠中显著更多。4. 通过测序和蛋白质免疫印迹分析发现，p29/6.8组分水平的显著差异是由碳酸酐酶III引起的。