气相色谱-质谱联用法测定人尿液中可替宁
[Determination of cotinine in human urine with gas chromatograph-mass spectrometry].
作者信息
Cao Min, Zhang Yi-lun, Jiang Chao-qiang, Lam Tai-hing, Cheng Kar-keung, Zhang Wei-sen
机构信息
Guangzhou Monitoring Centre of Occupational Health, Guangzhou 510620 China.
出版信息
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2006 Jan;24(1):39-41.
OBJECTIVE
To establish the method of gas chromatograph-mass spectrometry (GC-MS) for the determination of the cotinine (COT) in human urine.
METHODS
The conjugated trans-3'-hydroxycotinine (THOC) and COT were hydrolyzed in human urine with beta-glucuronidase. The composition of COT was extracted with the mixture of dichloromethane and n-butyl acetate (2:1) and was separated with HP-5MS fused-silica capillary column. The GC-MS was used for determining its content.
RESULTS
The monitoring limit of this method was 0.02 microg/L. Its recovery rate was higher than 90%, Its accuracy rate was 4.30%. It was used for the determination of the cotinine in human urine in Guangzhou Biological Bank the Elderly Cohort.
CONCLUSION
The GC-MS method is a good microanalysis for monitoring the cotinine in human urine rapidly and accurately with little background disturbance. It has been applied in our Guangzhou Cohort Study for determining cotinine in human urine.
目的
建立气相色谱-质谱联用法（GC-MS）测定人尿液中可替宁（COT）的方法。
方法
人尿液中的结合型反式-3'-羟基可替宁（THOC）和COT用β-葡萄糖醛酸酶水解。用二氯甲烷和乙酸正丁酯（2:1）的混合液萃取COT的成分，并用HP-5MS熔融石英毛细管柱进行分离。采用GC-MS测定其含量。
结果
该方法的检测限为0.02μg/L。回收率高于90%，准确率为4.30%。用于广州生物银行老年队列人尿液中可替宁的测定。
结论
GC-MS法是一种良好的微量分析方法，能快速、准确地监测人尿液中的可替宁，背景干扰小。已应用于我们的广州队列研究中测定人尿液中的可替宁。
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