Chang Fu-hou, Hu Ting-mao
Department of Pharmacology, Inner Mongolia Medical College, Huhhot 010059, China.
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2006 Feb;24(2):85-7.
To investigate the influence of the smoke tar on the expression of aromatic hydrocarbon receptor (AHR) and the cytochrome P4501Al (CYP1A1) gene of mice lungs.
The smoke tar of 5.29, 10.58 and 15.87 mg/kg was administered intraperitoneally in mice respectively. RNA of mice lungs was got with RNA kit. RT-PCR technique was used for determining AHR and CYP1A1 gene expression with beta-actin as control.
The AHR gene expression level was (0.554 +/- 0.023) for the mice intraperitoneally administered with 5.29 mg/kg smoke tar for 72 hours with the significant difference in gene expression level compared with the Tween-80 group (0.484 +/- 0.045) (P < 0.05). The AHR gene expression levels were (0.555 +/- 0.014), (0.606 +/- 0.051), and (0.566 +/- 0.014), (0.684 +/- 0.069) for the mice intraperitoneally administered with 10.58 and 15.87 mg/kg smoke tar for 48 hours and 72 hours respectively with the significant difference in gene expression level compared with the Tween-80 group (0.486 +/- 0.060, 0.484 +/- 0.045) (P < 0.05, P < 0.01). The CYP1Al gene expression levels were (1.535 +/- 0.021), (1.643 +/- 0.046) and (1.624 +/- 0.056), (1.739 +/- 0.038) respectively with the significant difference compared with the Tween-80 group (l.436 +/- 0.016, 1.404 +/- 0.036) (P < 0.01).
The smoke tar can regulate up the expression of AHR and CYP1A1 gene at a certain dosage and time. The regulation of the smoke tar for the expression of AHR was earlier than for that of CYP1A1.
探讨烟焦油对小鼠肺组织芳烃受体（AHR）及细胞色素P4501A1（CYP1A1）基因表达的影响。
分别以5.29、10.58和15.87mg/kg的烟焦油腹腔注射小鼠。用RNA试剂盒提取小鼠肺组织RNA。采用RT-PCR技术，以β-肌动蛋白为对照，检测AHR和CYP1A1基因表达。
腹腔注射5.29mg/kg烟焦油72小时的小鼠，AHR基因表达水平为（0.554±0.023），与吐温-80组（0.484±0.045）相比，基因表达水平有显著差异（P<0.05）。腹腔注射10.58mg/kg和15.87mg/kg烟焦油48小时和72小时的小鼠，AHR基因表达水平分别为（0.555±0.014）、（0.606±0.051）和（0.566±0.014）、（0.684±0.0
