[活性氧在氯化锰诱导PC12细胞凋亡中的作用机制]

[Mechanism of reactive oxygen species in manganese chloride-induced apoptosis in PC12 cells].

作者信息

Zeng Ji-ping, Wang Li-xiang, Xia Wen, Hu Xiao-yan, Kong Feng, Wu Wei-fang, Cui Xing

机构信息

Department of Biochemistry, School of Medicine, Shandong University, Jinan 250012, China.

出版信息

Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2006 Mar;24(3):157-60.

PMID:16600136

Abstract

OBJECTIVE

To explore the mechanism of reactive oxygen species (ROS) in manganese chloride (MnCl(2))-induced apoptosis in PC12 cells.

METHODS

The model that MnCl(2) induced apoptosis in PC12 cells was established. The apoptotic effect of MnCl(2) on PC12 cells was analyzed with the MTT, the flow cytometry and the DNA fragmentation. The production of ROS and ATP in MnCl(2)-induced apoptosis of PC12 cells was examined. The influence of MnCl(2) on the expression of bcl-xl, bax and the activity of Caspase 3 was also analyzed.

RESULTS

MnCl(2) triggered PC12 cells apoptosis in a dose-and time-dependent manner (P < 0.01). The rate of apoptosis was significantly increased (P < 0.01) when MnCl(2) of 2 mmol/L induced PC12 cells for 36 hours. The production of ROS was increased (P < 0.001) and the quantity of ATP was decreased (P < 0.01) in PC12 cells with the same inducement of MnCl(2). The expression of bcl-xl was inhibited and the bax was activated in this process (P < 0.01). Caspase 3 was also activated (P < 0.01).

CONCLUSION

MnCl(2) induces apoptosis of PC12 cells, which is related to the increase of ROS, the inhibition of the mitochondria and the activation of Caspase 3.

摘要

目的

探讨活性氧（ROS）在氯化锰（MnCl₂）诱导PC12细胞凋亡中的作用机制。

方法

建立MnCl₂诱导PC12细胞凋亡的模型。采用MTT法、流式细胞术及DNA片段化分析MnCl₂对PC12细胞的凋亡作用。检测MnCl₂诱导PC12细胞凋亡过程中ROS的产生及ATP的含量。分析MnCl₂对bcl-xl、bax表达及Caspase 3活性的影响。

结果

MnCl₂以剂量和时间依赖性方式诱导PC12细胞凋亡（P < 0.01）。2 mmol/L MnCl₂诱导PC12细胞36小时后，凋亡率显著增加（P < 0.01）。相同诱导条件下，PC12细胞内ROS产生增加（P < 0.001），ATP含量降低（P < 0.01）。此过程中bcl-xl表达受抑制，bax被激活（P < 0.01）。Caspase 3也被激活（P < 0.01）。