人纤维蛋白原在单壁碳纳米管薄膜上的吸附

Human fibrinogen adsorption onto single-walled carbon nanotube films.

作者信息

Song Li, Meng Jie, Zhong Jun, Liu Lifeng, Dou Xinyuan, Liu Dongfang, Zhao Xiaowei, Luo Sudong, Zhang Zengxing, Xiang Yanjuan, Xu Haiyan, Zhou Weiya, Wu Ziyu, Xie Sishen

机构信息

Beijing National Laboratory for Condensed Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing 100080, China.

出版信息

Colloids Surf B Biointerfaces. 2006 Apr 15;49(1):66-70. doi: 10.1016/j.colsurfb.2005.12.003. Epub 2006 Jan 18.

DOI:10.1016/j.colsurfb.2005.12.003

PMID:16600577

Abstract

The adsorption behavior of human fibrinogen (Hfg) on single-walled carbon nanotube (SWNT) films was investigated using scanning electron microscopy (SEM) and near edge X-ray absorption fine structure (NEXAFS) spectroscopy. It was shown in the SEM images that fibrinogen was adsorbed strongly on the surface of SWNT when the samples were incubated in the Hfg solutions for 10 min. The dependence of adsorption on the concentration of fibrinogen was also investigated and it was found that adsorption increased with increasing concentration. In order to further explore the adsorption of fibrinogen on SWNT surface, NEXAFS spectra were obtained at the N K-edge and the C K-edge. The results confirmed the conclusion regarding the dependence of adsorption on fibrinogen concentration. It is demonstrated that, due to its high sensitivity to the surface elements, NEXAFS spectroscopy is a powerful tool to investigate the adsorption of fibrinogen on SWNT films.

摘要

利用扫描电子显微镜（SEM）和近边X射线吸收精细结构（NEXAFS）光谱研究了人纤维蛋白原（Hfg）在单壁碳纳米管（SWNT）薄膜上的吸附行为。SEM图像显示，当样品在Hfg溶液中孵育10分钟时，纤维蛋白原强烈吸附在SWNT表面。还研究了吸附对纤维蛋白原浓度的依赖性，发现吸附量随浓度增加而增加。为了进一步探究纤维蛋白原在SWNT表面的吸附情况，在N K边和C K边获得了NEXAFS光谱。结果证实了关于吸附对纤维蛋白原浓度依赖性的结论。结果表明，由于NEXAFS光谱对表面元素具有高灵敏度，它是研究纤维蛋白原在SWNT薄膜上吸附的有力工具。