单壁碳纳米管对血浆蛋白功能及血管假体电位的影响。

Effects of single-walled carbon nanotubes on the functions of plasma proteins and potentials in vascular prostheses.

作者信息

Meng Jie, Song Li, Xu Haiyan, Kong Hua, Wang Chaoying, Guo Xiaotian, Xie Sishen

机构信息

Institute of Basic Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

出版信息

Nanomedicine. 2005 Jun;1(2):136-42. doi: 10.1016/j.nano.2005.03.003.

DOI:10.1016/j.nano.2005.03.003

PMID:17292070

Abstract

BACKGROUND

Nonwoven single-walled nanotubes (SWNTs) are novel materials with a nanotopography macroscopic surface and pure-carbon composition, which may serve as coatings on implants in blood-contact environments.

METHODS

The adsorption behavior of albumin, fibrinogen, and fresh plasma on SWNT membranes was studied using scanning electron microscopy (SEM), energy-dispersive x-ray, and direct enzyme-linked immunosorbent assay, respectively. Platelet adhesion and activation were investigated in vitro using SEM observation, and via flow cytometry analysis by PAC-1, which binds to GPIIb/IIIa expressed on activated platelets.

RESULTS

Nonwoven SWNTs clearly displayed greater adsorption preference for fibrinogen than for albumin. However, adhered platelets were not observed by SEM. Results obtained from flow cytometry analysis further proved very low expression of GPIIb/IIIa on platelets caused by nonwoven SWNTs.

CONCLUSION

The function of adsorbed fibrinogen to mediate platelet recognition, adhesion, activation, and aggregation was significantly suppressed, which induced extremely low levels of platelet adhesion and activation.

摘要