Pinto V B, Peacock J S
Department of Microbiology and Immunology, University of Miami School of Medicine, FL 33101.
Mol Immunol. 1991 Nov;28(11):1311-4. doi: 10.1016/0161-5890(91)90018-f.
We wished to determine whether tolerized cells are able to process and present antigen based on our hypothesis that tolerized B cells be unable to function in the normal capacity as antigen-presenting cells if they are to remain the tolerant state. Results in this study show that the ability of the murine lymphoma model for immature B cells, CH31, to process pigeon cytochrome c was greatly down-regulated when cultured in the presence of rabbit anti-mouse IgM. In contrast, the same anti-IgM treatment had no significant effect on the antigen-presenting cell function of the lymphoma model for mature B cells, CH112. Presentation of CNBr-cleaved fragments of pigeon cytochrome c by either CH31 or CH12 cells was not affected by the antibody treatment. Furthermore, CH31 cells pre-incubated with pigeon cytochrome c were not subject to the anti-IgM inhibition of the antigen presentation. These observations suggest that pertubation of surface immunoglobulin molecules on CH31 immature B cells causes down-regulation of their antigen-processing machinery.
基于我们的假设，即如果耐受的B细胞要保持耐受状态，它们就无法以正常能力作为抗原呈递细胞发挥作用，我们希望确定耐受细胞是否能够加工和呈递抗原。本研究结果表明，在存在兔抗小鼠IgM的情况下培养时，用于未成熟B细胞的小鼠淋巴瘤模型CH31加工鸽细胞色素c的能力被大大下调。相比之下，相同的抗IgM处理对成熟B细胞淋巴瘤模型CH112的抗原呈递细胞功能没有显著影响。CH31或CH12细胞对溴化氰裂解的鸽细胞色素c片段的呈递不受抗体处理的影响。此外，预先用鸽细胞色素c孵育的CH31细胞不受抗IgM对抗原呈递的抑制作用。这些观察结果表明，CH31未成熟B细胞表面免疫球蛋白分子的扰动会导致其抗原加工机制的下调。
