Tátrai Erzsébet, Brózik Marta, Kováciková Zuzana, Horváth Magdolna
Fodor József National Center for Public Health, Budapest, Hungary.
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2005 Dec;149(2):357-61.
The in vitro effect of stone-wool has been studied in primary cultures of pulmonary alveolar macrophages (AM) and type II pneumocytes (T2) by morphological, biochemical and immunological methods. UICC crocidolite was applied as a positive control. Although stone-wool brought about frustrated phagocytosis, it did not induce serious membrane damage, whereas crocidolite gave rise to very severe membrane alterations. Stone-wool significantly reduced the activity of Cu,Zn/superoxide dismutase (SOD) in alveolar macrophages and significantly decreased the activity of gamma-glutamyl transpeptidase (GGT) in pneumocytes type II. Crocidolite, on the other hand, decreased the activity of all enzymes (glutathione peroxidase - GSH-Px, glutathione reductase - GSH-Rd) of glutathione metabolism in alveolar macrophages. It decreased the activity of all enzymes in pneumocytes type II except for Cu,Zn/SOD. After exposure to stone-wool, the production of inflammatory proteins, macrophage chemoattractant protein-1 (MCP-1) and macrophage inhibitory protein-1alpha (MIP-1alpha) increased in both cultured cells but did not reach the level induced by crocidolite. Although this study provided a useful insight in the toxicity of the stone-wool, we can not draw the conclusions how the intact pulmonary tissue may respond on the exposure to these fibres, exclusively based on the in vitro tests.
已通过形态学、生物化学和免疫学方法，在肺泡巨噬细胞（AM）和II型肺细胞（T2）的原代培养物中研究了石棉绒的体外效应。将国际癌症研究机构（UICC）的青石棉用作阳性对照。尽管石棉绒导致吞噬作用受阻，但并未引起严重的膜损伤，而青石棉则引起非常严重的膜改变。石棉绒显著降低了肺泡巨噬细胞中铜锌超氧化物歧化酶（SOD）的活性，并显著降低了II型肺细胞中γ-谷氨酰转肽酶（GGT）的活性。另一方面，青石棉降低了肺泡巨噬细胞中谷胱甘肽代谢的所有酶（谷胱甘肽过氧化物酶-GSH-Px、谷胱甘肽还原酶-GSH-Rd）的活性。它降低了II型肺细胞中除铜锌超氧化物歧化酶外的所有酶的活性。暴露于石棉绒后，两种培养细胞中炎症蛋白、巨噬细胞趋化蛋白-1（MCP-1）和巨噬细胞抑制蛋白-1α（MIP-1α）的产生均增加，但未达到青石棉诱导的水平。尽管这项研究为石棉绒的毒性提供了有用的见解，但仅基于体外试验，我们无法得出完整肺组织在接触这些纤维时可能如何反应的结论。
