Cardinali Giorgia, Kovacs Daniela, Maresca Vittoria, Flori Enrica, Dell'Anna Maria Lucia, Campopiano Antonella, Casciardi Stefano, Spagnoli Giuseppe, Torrisi Maria Rosaria, Picardo Mauro
San Gallicano Dermatological Institute, Via San Gallicano 25/A, 00153 Rome, Italy.
Exp Mol Pathol. 2006 Aug;81(1):31-41. doi: 10.1016/j.yexmp.2005.10.001. Epub 2005 Dec 13.
In this study, we analyzed the effects of synthetic vitreous fibers (SVFs) on a mesothelial (MeT5A) and a fibroblast cell line (NIH3T3), compared to those exerted by crocidolite asbestos fibers. SVFs (glass wool, rock wools) do not induce significant changes in cell mortality, whereas crocidolite asbestos fibers caused a dose-dependent cytotoxicity. We investigated the correlation between the fiber-induced cytotoxicity and the extent and type of interaction of the fibers with the cell surface, and we observed that SVFs, unlike crocidolite asbestos fibers, establish few and weak interactions. Moreover, after internalization, crocidolite asbestos fibers are often found free in the cytoplasm, whereas glass wool fibers are mainly localized inside cytoplasmic vacuoles. After treatments, we also detected signs of oxidative stress, revealed by an increased reactive oxygen species (ROS) production and by an induction of superoxide dismutase (SOD) activity. The lipoperoxidative damage was characterized by a decrease in polyunsaturated fatty acids (PUFA), an increase in the content of thiobarbituric reactive species (TBARS) and a consumption of vitamin E, as a lipophilic antioxidant. Furthermore, we investigated the effect of fiber exposure on cell proliferation. and it was found that, unlike crocidolite asbestos fibers, SVFs did not induce a significant increase in DNA synthesis.
在本研究中，我们分析了合成玻璃纤维（SVFs）对间皮细胞系（MeT5A）和成纤维细胞系（NIH3T3）的影响，并与青石棉纤维的作用进行了比较。SVFs（玻璃棉、岩棉）不会引起细胞死亡率的显著变化，而青石棉纤维会导致剂量依赖性细胞毒性。我们研究了纤维诱导的细胞毒性与纤维与细胞表面相互作用的程度和类型之间的相关性，并且我们观察到，与青石棉纤维不同，SVFs建立的相互作用很少且较弱。此外，内化后，青石棉纤维经常在细胞质中游离存在，而玻璃棉纤维主要定位于细胞质空泡内。处理后，我们还检测到了氧化应激的迹象，表现为活性氧（ROS）产生增加和超氧化物歧化酶（SOD）活性诱导。脂质过氧化损伤的特征是多不饱和脂肪酸（PUFA）减少、硫代巴比妥酸反应性物质（TBARS）含量增加以及作为亲脂性抗氧化剂的维生素E消耗。此外，我们研究了纤维暴露对细胞增殖的影响，发现与青石棉纤维不同，SVFs不会诱导DNA合成显著增加。
