使用电化学方法研究铅中毒并接受铂治疗的患者血清中金属硫蛋白的含量。

Using of electrochemical methods for studying of metallothionein content in the human blood serum of a patient poisoned by lead and treated by platinum.

作者信息

Petrlová Jitka, Blastík Ondrej, Průsa Richard, Kukacka Jirí, Potĕsil David, Mikelová Radka, Adam Vojtĕch, Zehnálek Josef, Kizek René

机构信息

Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University of Agriculture and Forestry, Zemedelská 1, Brno, CZ-613 00, Czech Republic.

出版信息

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2005 Dec;149(2):485-8.

PMID:16601816

Abstract

Metallothioneins belong to the group of intracellular, high molecular and cysteine-rich proteins whose content increase with increasing concentration of a heavy metal. Here we applied the adsorptive transfer stripping differential pulse voltammetry Brdicka reaction for the determination of metallothionein in human blood serum of patient poisoned by lead and/or treated by platinum. The increased metallothionein concentrations in both cases were observed.

摘要

金属硫蛋白属于细胞内、高分子量且富含半胱氨酸的蛋白质类别，其含量会随着重金属浓度的增加而升高。在此，我们运用吸附转移溶出微分脉冲伏安法的布迪卡反应来测定铅中毒和/或接受铂治疗患者的人血清中的金属硫蛋白。在这两种情况下均观察到金属硫蛋白浓度升高。