适用于膜片钳研究的豚鼠胆囊上皮细胞原代培养物。
Primary cultures of guinea-pig gall-bladder epithelial cells suitable for patch-clamp studies.
作者信息
O'Brien J A, James P S, Torre M
机构信息
Department of Cell Biology, AFRC Institute of Animal Physiology & Genetics Research, Babraham, Cambridge.
出版信息
Exp Physiol. 1991 Sep;76(5):807-10. doi: 10.1113/expphysiol.1991.sp003546.
Abstract
Cells derived from explants of guinea-pig gall-bladder maintained in culture for up to 14 days, exhibit a network of cytoskeletal filaments that are labelled by an anti-cytokeratin antibody, but not by an anti-desmin antibody, suggesting that they are of epithelial origin. These cells proved suitable for patch-clamp studies and by using this method channels selective for K+ and Cl- were observed.
摘要
源自豚鼠胆囊外植体并在培养中维持长达14天的细胞，呈现出由抗细胞角蛋白抗体标记但抗结蛋白抗体未标记的细胞骨架丝网络，表明它们起源于上皮细胞。这些细胞被证明适用于膜片钳研究，并且通过使用该方法观察到了对K⁺和Cl⁻具有选择性的通道。
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