Couderc B, Prats H, Bayard F, Amalric F
Centre de Recherche de Biochimie, Hôpital Rangueil, Toulouse, France.
Cell Regul. 1991 Sep;2(9):709-18. doi: 10.1091/mbc.2.9.709.
Normal adult bovine aortic endothelial cells were infected with various recombinant retroviruses expressing one, two, or three human basic fibroblast growth factor (bFGF) proteins normally synthesized by an alternative use of translation initiation codons. We show here that the constitutive expression of the AUG-initiated from (18 kDa) leads the transfected cells to form colonies in soft agar. The expression of the high molar weight (HMW) forms (22.5 and 21 kDa) initiated at one of the two CUG initiation codons allows cell immortalization, whereas the tumorigenic potential is reached when the three forms are constitutively expressed. Furthermore, we provide evidence that constitutive expression of (HMW) bFGF forms has a down-regulation effect on bFGF synthesis from the gene naturally active in parental endothelial cells.
正常成年牛主动脉内皮细胞被多种重组逆转录病毒感染，这些病毒表达一种、两种或三种人类碱性成纤维细胞生长因子（bFGF）蛋白，这些蛋白通常通过交替使用翻译起始密码子来合成。我们在此表明，由AUG起始的（18 kDa）的组成型表达导致转染细胞在软琼脂中形成集落。在两个CUG起始密码子之一起始的高摩尔质量（HMW）形式（22.5和21 kDa）的表达可使细胞永生化，而当三种形式组成型表达时则具有致瘤潜力。此外，我们提供的证据表明，（HMW）bFGF形式的组成型表达对亲本内皮细胞中天然活跃基因的bFGF合成具有下调作用。
