苏云金芽孢杆菌Cry1Ac毒素在T7噬菌体上的功能展示
Functional display of Bacillus thuringiensis Cry1Ac toxin on T7 phage.
作者信息
Pacheco Sabino, Gómez Isabel, Sato Ryoichi, Bravo Alejandra, Soberón Mario
机构信息
Departamento de Microbiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos.
出版信息
J Invertebr Pathol. 2006 May;92(1):45-9. doi: 10.1016/j.jip.2006.02.007. Epub 2006 Apr 17.
The Cry1Ac toxin from Bacillus thuringiensis was displayed on the surface of T7 phage. The cry1Ac gene was fused to the C-terminal end of T7-10B capsid protein and displayed on the surface of T7 phage as revealed by Western blot analysis of the purified phage particles. The T7-Cry1Ac phages retained toxicity against Manduca sexta larvae. We demonstrated that the T7-Cry1Ac phage interacts with Cry1Ac receptors present in M. sexta BBMV either in solution or in overlay binding assays.
来自苏云金芽孢杆菌的Cry1Ac毒素展示在T7噬菌体表面。cry1Ac基因与T7 - 10B衣壳蛋白的C末端融合，并通过对纯化的噬菌体颗粒进行蛋白质免疫印迹分析表明其展示在T7噬菌体表面。T7 - Cry1Ac噬菌体对烟草天蛾幼虫保持毒性。我们证明，无论是在溶液中还是在覆盖结合试验中，T7 - Cry1Ac噬菌体都能与烟草天蛾刷状缘膜囊泡（BBMV）中存在的Cry1Ac受体相互作用。
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