Karlova Rumyana, Weemen-Hendriks Mieke, Naimov Samir, Ceron Jairo, Dukiandjiev Stefan, de Maagd Ruud A
Department of Plant Physiology and Molecular Biology, University of Plovdiv, 4000 Plovdiv, Bulgaria.
J Invertebr Pathol. 2005 Feb;88(2):169-72. doi: 10.1016/j.jip.2004.11.004. Epub 2005 Jan 5.
We investigated the role of domain III of Bacillus thuringiensis delta-endotoxin Cry1Ac in determining toxicity against Heliothis virescens. Hybrid toxins, containing domain III of Cry1Ac with domains I and II of Cry1Ba, Cry1Ca, Cry1Da, Cry1Ea, and Cry1Fb, respectively, were created. In this way Cry1Ca, Cry1Fb, and to a lesser extent Cry1Ba were made considerably more toxic.
我们研究了苏云金芽孢杆菌δ-内毒素Cry1Ac的结构域III在决定对棉铃虫毒性方面的作用。分别构建了含有Cry1Ac的结构域III与Cry1Ba、Cry1Ca、Cry1Da、Cry1Ea和Cry1Fb的结构域I和II的杂合毒素。通过这种方式，Cry1Ca、Cry1Fb以及在较小程度上Cry1Ba的毒性显著增强。
