Baek Kwang-Hyun, Skinner Daniel Z, Ling Peng, Chen Xianming
Department of Crop and Soil Sciences, 210 Johnson Hall, Washington State University, Pullman, WA 99164-6420, USA.
Genome. 2006 Mar;49(3):209-18. doi: 10.1139/g05-102.
The genomic structure of a manganese superoxide dismutase (MnSOD) gene in wheat was elucidated by sequencing a clone from a BAC library of a stripe rust resistant wheat line. The clone was identified by hybridization with a wheat MnSOD cDNA. The gene consisted of 6 exons interrupted by 5 introns with a total length of 4770 nucleotides from the start codon to the termination codon. The wheat MnSOD gene was the longest among those sequenced from plant species. The transcription initiation site was preceded by a G+C-rich promoter without a TATA or CAAT box. The promoter contained many putative cis-acting regulatory elements, including an abscisic acid (ABA)-responsive element, a stress-responsive element, and a GC-repeat, as well as several other structural features in common with the promoter of the rice MnSOD gene. A Stowaway-like transposable element was found in intron 5 of the wheat MnSOD gene, but further investigation revealed the transposable element was not present in all copies of the MnSOD genes.
通过对一个抗条锈病小麦品系BAC文库中的一个克隆进行测序，阐明了小麦中锰超氧化物歧化酶（MnSOD）基因的基因组结构。该克隆通过与小麦MnSOD cDNA杂交进行鉴定。该基因由6个外显子和5个内含子组成，从起始密码子到终止密码子全长4770个核苷酸。小麦MnSOD基因是已测序植物物种中最长的。转录起始位点之前是一个富含G+C的启动子，没有TATA盒或CAAT盒。该启动子包含许多推定的顺式作用调控元件，包括一个脱落酸（ABA）响应元件、一个胁迫响应元件和一个GC重复序列，以及一些与水稻MnSOD基因启动子共有的其他结构特征。在小麦MnSOD基因的内含子5中发现了一个类似Stowaway的转座元件，但进一步研究表明，并非所有MnSOD基因拷贝中都存在该转座元件。
