Xu Tao, Zhao Bao-Cun, Ge Rong-Chao, Shen Yin-Zhu, Huang Zhan-Jing
The College of Biology, Hebei Normal University, Shijiazhuang 050016, China.
Sheng Wu Gong Cheng Xue Bao. 2006 Mar;22(2):211-4.
The Tagsk1 (Triticum asetium L. glycogen synthase kinase 1) gene derived from the genome of wheat salt-tolerance mutant RH8706-49 was cloned by PCR. The special primers designed according to full length cDNA sequence of Tagsk1 (AF525086). A binary expression vector pBI121-gsk1 containing Gus and Tagsk1 was constructed. And pBI121-gsk1 was introduced into the callus induced from mature embryos of salt-sensitive wheat H8706-34 and cv. China Spring by particle bombardment. The transformed callus were screened by Kanamycin and 0.5% NaCl. The salt-tolerance callus were obtained, which showed higher ability of salt-tolerance and could diffirentiate roots and buds on the medium containing 0.5% NaCl.
通过PCR克隆了源自耐盐小麦突变体RH8706 - 49基因组的Tagsk1（普通小麦糖原合酶激酶1）基因。根据Tagsk1（AF525086）的全长cDNA序列设计了特异性引物。构建了包含Gus和Tagsk1的二元表达载体pBI121 - gsk1。通过粒子轰击将pBI121 - gsk1导入盐敏感小麦H8706 - 34和中国春品种成熟胚诱导的愈伤组织中。用卡那霉素和0.5% NaCl对转化的愈伤组织进行筛选。获得了耐盐愈伤组织，其在含0.5% NaCl的培养基上表现出较高的耐盐能力，并且能够分化出根和芽。
