大川芎丸对脑缺血大鼠血管内皮生长因子表达的影响

[Effect of dachuanxiongwan on the expression of vascular epithelial growth factor in rats with cerebral ischemia].

作者信息

Zhang Zhi-Jian, Kong Shuang-Yan, Zhou Dong, He Li

机构信息

Department of neurology, West China Hospital, Sichuan University, Chengdu 610041, China

出版信息

Sichuan Da Xue Xue Bao Yi Xue Ban. 2006 Mar;37(2):246-9.

PMID:16608086

Abstract

OBJECTIVE

To study the effect of Dachuanxiongwan on the expression of vascular epithelial growth factor in the rats after focal cerebral ischemia and reperfusion.

METHODS

The ischemia and reperfusion model was established by blocking the middle cerebral artery of the rats with a nylon thread for two hours. Twenty male Sprague-Dawley rats with middle cerebral artery occlusion (MCAO) were randomly divided into two groups. One of the group of rats were administered with DCXW, 4.13 g/kg x d, twice a day for 3 days by gavaging, while other group were given the same volume of saline as a control. Seventy-two hours after reperfusion, the neuropathological function of the rats were evaluated according to Longa. The rats were then sacrificed and the samples of cerebrum were processed and embedded in paraffin and cut into sections. The expression of VEGF was determined by immunocytochemistry.

RESULTS

The score of the neuropathological function of the DCXW treated group (1.5 +/- 0.71) was lower than that of the control group (2.3 +/- 0.82, P = 0.032). More VEGF-positive cells were found in the rats treated with DCXW than those in the control group (P = 0.000).

CONCLUSION

DCXW upgrades the expression of VEGF and may have a positive effect on the cerebrum following ischemia and reperfusion.

摘要

目的

研究大川芎丸对大鼠局灶性脑缺血再灌注后血管内皮生长因子表达的影响。

方法

采用尼龙线阻塞大鼠大脑中动脉2小时建立缺血再灌注模型。将20只雄性Sprague-Dawley大鼠大脑中动脉闭塞（MCAO）模型随机分为两组。一组大鼠灌胃给予大川芎丸，4.13 g/kg×d，每日2次，连续3天，另一组给予等体积生理盐水作为对照。再灌注72小时后，根据Longa法评估大鼠神经病理功能。然后处死大鼠，取大脑样本进行处理，石蜡包埋并切片。采用免疫细胞化学法检测血管内皮生长因子（VEGF）的表达。