Yin Jun-xiang, Tian Jin-zhou, Shi Jing, Cheng Long, Wang Yong-yan
Institute of Geriatrics, Beijing University of Chinese Medicine, Beijing 100700, China.
Zhongguo Zhong Yao Za Zhi. 2006 Dec;31(23):1983-6.
To explore the influence of one Chinese herbal extract complex (GETO) on the expression of corticotropin-releasing factor(CRF) and protein kinasec(PKC) proteins of the hippocampus in middle cerebral artery orilusion(MCAO) rats.
All rats were subjected to MCAO by nylon thread, except the sham-group rats. Rats were divied into four groups: sham-group, cerebral ischemia model-group, GETO-group(6. 1 g x kg(-1) x d(-1) )and Duxil-group (7.3 mg x kg(-1) x d(-1)). Using immunohistochemistry technique we measured the expression quantity of CRF and PKC protein in hippocampus of MCAO rats at 2 h,6 h and 24 h after reperfusion, contrasted to Duxil.
CRF: There were lots of positive and deeper dyeing neurons in hippocampus of model-group rats, while there were a few of positive and lighter dyeing neurons in sham-group, GETO-group and Duxil-group. The positive expression areas of CRF protein in hippocampus of model-group was significantly bigger than that of sham-group, GETO -group and Duxil-group respectively( P <0. 01). PKC: There were a great number of denser positive granules in hippocampus of model-group rats, while there were a few of scattered positive granules in sham-group, GETO-group and Duxil-group. The positive expression areas of CRF protein in hippocampus of model-group was significantly bigger than that of sham-group, GETO-group and Duxil-group respectively( P < 0. 01). At the same time there was not significant difference about the expression of CRF and PKC protein between GETO group and Duxil-group.
The high expression of CRF and PKC induced by cerebral ischemia may be one important factor that resulted in the delayed neuronal death in hippocampus. GETO can down-regulate the expression of CRF and PKC induced by cerebral ischemia, which may be one of the mechanisms that the Chinese herbal extract complex, protect cerebral ischemic injury.
探讨一种中药提取物复合物（GETO）对大脑中动脉闭塞（MCAO）大鼠海马促肾上腺皮质激素释放因子（CRF）和蛋白激酶C（PKC）蛋白表达的影响。
除假手术组大鼠外，所有大鼠均采用尼龙线进行MCAO。将大鼠分为四组：假手术组、脑缺血模型组、GETO组（6.1 g·kg⁻¹·d⁻¹）和都可喜组（7.3 mg·kg⁻¹·d⁻¹）。采用免疫组织化学技术，于再灌注后2小时、6小时和24小时测量MCAO大鼠海马中CRF和PKC蛋白的表达量，并与都可喜组进行对比。
CRF：模型组大鼠海马中有大量阳性且染色较深的神经元，而假手术组、GETO组和都可喜组中只有少数阳性且染色较浅的神经元。模型组大鼠海马中CRF蛋白的阳性表达面积分别显著大于假手术组、GETO组和都可喜组（P<0.01）。PKC：模型组大鼠海马中有大量密集的阳性颗粒，而假手术组、GETO组和都可喜组中只有少数散在的阳性颗粒。模型组大鼠海马中CRF蛋白的阳性表达面积分别显著大于假手术组、GETO组和都可喜组（P<0.0）。同时，GETO组和都可喜组之间CRF和PKC蛋白的表达无显著差异。
脑缺血诱导的CRF和PKC高表达可能是导致海马神经元延迟性死亡的重要因素之一。GETO可下调脑缺血诱导的CRF和PKC表达，这可能是该中药提取物复合物保护脑缺血损伤的机制之一。
