中药提取物复合物对大脑中动脉闭塞大鼠海马促肾上腺皮质激素释放因子及蛋白激酶C蛋白表达的影响

[Influence of Chinese herbal extract complex on expression of corticotropin-releasing factor and protein kinasec protein in hippocampus of middle cerebral artery occlusion rats].

作者信息

Yin Jun-xiang, Tian Jin-zhou, Shi Jing, Cheng Long, Wang Yong-yan

机构信息

Institute of Geriatrics, Beijing University of Chinese Medicine, Beijing 100700, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2006 Dec;31(23):1983-6.

PMID:17348196

Abstract

OBJECTIVE

To explore the influence of one Chinese herbal extract complex (GETO) on the expression of corticotropin-releasing factor(CRF) and protein kinasec(PKC) proteins of the hippocampus in middle cerebral artery orilusion(MCAO) rats.

METHOD

All rats were subjected to MCAO by nylon thread, except the sham-group rats. Rats were divied into four groups: sham-group, cerebral ischemia model-group, GETO-group(6. 1 g x kg(-1) x d(-1) )and Duxil-group (7.3 mg x kg(-1) x d(-1)). Using immunohistochemistry technique we measured the expression quantity of CRF and PKC protein in hippocampus of MCAO rats at 2 h,6 h and 24 h after reperfusion, contrasted to Duxil.

RESULT

CRF: There were lots of positive and deeper dyeing neurons in hippocampus of model-group rats, while there were a few of positive and lighter dyeing neurons in sham-group, GETO-group and Duxil-group. The positive expression areas of CRF protein in hippocampus of model-group was significantly bigger than that of sham-group, GETO -group and Duxil-group respectively( P <0. 01). PKC: There were a great number of denser positive granules in hippocampus of model-group rats, while there were a few of scattered positive granules in sham-group, GETO-group and Duxil-group. The positive expression areas of CRF protein in hippocampus of model-group was significantly bigger than that of sham-group, GETO-group and Duxil-group respectively( P < 0. 01). At the same time there was not significant difference about the expression of CRF and PKC protein between GETO group and Duxil-group.

CONCLUSION

The high expression of CRF and PKC induced by cerebral ischemia may be one important factor that resulted in the delayed neuronal death in hippocampus. GETO can down-regulate the expression of CRF and PKC induced by cerebral ischemia, which may be one of the mechanisms that the Chinese herbal extract complex, protect cerebral ischemic injury.

摘要

目的

探讨一种中药提取物复合物（GETO）对大脑中动脉闭塞（MCAO）大鼠海马促肾上腺皮质激素释放因子（CRF）和蛋白激酶C（PKC）蛋白表达的影响。

方法

除假手术组大鼠外，所有大鼠均采用尼龙线进行MCAO。将大鼠分为四组：假手术组、脑缺血模型组、GETO组（6.1 g·kg⁻¹·d⁻¹）和都可喜组（7.3 mg·kg⁻¹·d⁻¹）。采用免疫组织化学技术，于再灌注后2小时、6小时和24小时测量MCAO大鼠海马中CRF和PKC蛋白的表达量，并与都可喜组进行对比。

结果

CRF：模型组大鼠海马中有大量阳性且染色较深的神经元，而假手术组、GETO组和都可喜组中只有少数阳性且染色较浅的神经元。模型组大鼠海马中CRF蛋白的阳性表达面积分别显著大于假手术组、GETO组和都可喜组（P<0.01）。PKC：模型组大鼠海马中有大量密集的阳性颗粒，而假手术组、GETO组和都可喜组中只有少数散在的阳性颗粒。模型组大鼠海马中CRF蛋白的阳性表达面积分别显著大于假手术组、GETO组和都可喜组（P<0.0）。同时，GETO组和都可喜组之间CRF和PKC蛋白的表达无显著差异。