Chen Zhao-Bin, Zhang Chao-Wu, Xu Xin, Zhu Min, Wang Guo-Qing
Department of Medical Laboratory Technology, West China School of Public health, Sichuan University, Chengdu 610041, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2006 Mar;37(2):270-3.
To identify the best indicative bacteriophage in disinfection tests through comparing the resistance of bacteriophage T4, phiX174D, and f2 to glutaraldehyde.
The virucidal activities of glutaraldehyde against bacteriophage T4, phiX174D, and f2 were assessed with suspension tests along with neutralizer tests. The double-agar-layer plaque technique was used to detect the bacteriophage T4, phiX174D, and f2.
(1)In a condition of 3000 mg/L of glutaraldehyde and 20 minutes of contact or 6000 mg/L of glutaraldehyde and 5 minutes of contact, "disinfection" level for bacteriophage T4 was achieved, with the log10 inactivation value (LIV) or log10o reduction value (LRV) (=log10No-log10Nt) > or = 4. 00 log10. (2) In a condition of 2500 mg/L of glutaraldehyde and 20 minutes of contact or 5000 mg/L of glutaraldehyde and 5 minutes of contact, the LIV for bacteriophage phiX174D reached "disinfection" level; (3) In a condition of 4000 mg/L of glutaraldehyde and 40 minutes of contact or 8000 mg/L of glutaraldehyde and 10 minutes of contact, the LIV for bacteriophage f2 reached "disinfection" level.
Bacteriophage f2 and bacteriophage phiX174D has the strongest and weakest resistance to glutaraldehyde respectively.
通过比较噬菌体T4、phiX174D和f2对戊二醛的抗性，确定消毒试验中最佳指示噬菌体。
采用悬液试验和中和剂试验评估戊二醛对噬菌体T4、phiX174D和f2的杀病毒活性。采用双层琼脂平板法检测噬菌体T4、phiX174D和f2。
（1）在3000mg/L戊二醛作用20分钟或6000mg/L戊二醛作用5分钟的条件下，噬菌体T4达到“消毒”水平，对数10灭活值（LIV）或对数10减少值（LRV）（=log10No-log10Nt）≥4.00 log10。（2）在2500mg/L戊二醛作用20分钟或5000mg/L戊二醛作用5分钟的条件下，噬菌体phiX174D的LIV达到“消毒”水平；（3）在4000mg/L戊二醛作用40分钟或8000mg/L戊二醛作用10分钟的条件下，噬菌体f2的LIV达到“消毒”水平。
噬菌体f2和噬菌体phiX174D对戊二醛的抗性分别最强和最弱。
Zotero 插件