Zhou J, Crawford L, Sun X Y, Frazer I H
Department of Medicine, Princess Alexandra Hospital, Woolloongabba, Australia.
Gene. 1991 Nov 15;107(2):307-12. doi: 10.1016/0378-1119(91)90331-5.
Hygromycin B (Hy), an inhibitor of RNA translation, was shown to block the replication of vaccinia virus (VV) in cultured cell lines. Insertion of the Escherichia coli Hy resistance-encoding gene (hph) into the VV genome under control of early or late synthetic VV promoters could overcome inhibition of viral replication. When hph was inserted into VV in tandem with the human papillomavirus type 16 (HPV16) L1 open reading frame, hph recombinant viruses could be selected which expressed HPV16 L1.
潮霉素B（Hy）是一种RNA翻译抑制剂，已证明它能在培养的细胞系中阻断痘苗病毒（VV）的复制。将大肠杆菌潮霉素抗性编码基因（hph）在早期或晚期合成痘苗病毒启动子的控制下插入痘苗病毒基因组，可以克服对病毒复制的抑制。当hph与16型人乳头瘤病毒（HPV16）L1开放阅读框串联插入痘苗病毒时，可以筛选出表达HPV16 L1的hph重组病毒。
