传染性法氏囊病病毒的病毒蛋白2在酿酒酵母中的稳定合成。
Stable synthesis of viral protein 2 of infectious bursal disease virus in Saccharomyces cerevisiae.
作者信息
Jagadish M N, Laughton D L, Azad A A, Macreadie I G
机构信息
CSIRO, Division of Biomolecular Engineering, Parkville Laboratory, Victoria, Australia.
出版信息
Gene. 1991 Dec 15;108(2):275-9. doi: 10.1016/0378-1119(91)90445-h.
Viral protein 2 (VP2) from infectious bursal disease virus and its precursor polyprotein (N-VP2-VP4-VP3-C), in the absence of their native N-terminal region (19 amino acids), required fusion of yeast presequences for their stable synthesis in Saccharomyces cerevisiae [Jagadish et al., Gene 95 (1990) 179-186]. Restoration of the missing 19 aa resulted in stable synthesis of VP2, indicating the significance of the N-terminal region in protein stability.
传染性法氏囊病病毒的病毒蛋白2（VP2）及其前体多聚蛋白（N-VP2-VP4-VP3-C），在没有其天然N端区域（19个氨基酸）的情况下，需要与酵母前导序列融合才能在酿酒酵母中稳定合成[Jagadish等人，《基因》95（1990）179-186]。缺失的19个氨基酸的恢复导致VP2的稳定合成，表明N端区域对蛋白质稳定性的重要性。
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