通过电子自旋共振（ESR）光谱法对氯丙嗪进行定量分析。

Quantitative analysis of chlorpromazine by electron spin resonance (ESR) spectroscopy.

作者信息

Minakata K, Suzuki O, Ishikawa Y, Seno H, Asano M

机构信息

Department of Legal Medicine, Hamamatsu University, School of Medicine, Japan.

出版信息

Forensic Sci Int. 1991 Sep;50(2):167-77. doi: 10.1016/0379-0738(91)90148-c.

DOI:10.1016/0379-0738(91)90148-c

PMID:1660844

Abstract

A simple and sensitive method is described for quantitative analysis of chlorpromazine in blood, serum, urine and tissue homogenate. The chlorpromazine cation radical produced by adding perchloric acid and 2,3-dichloro-5,6-dicyano-p-benzoquinone to the sample can be detected by the ESR method at room temperature. The sensitivity limit is 10 ng, that is, 20 microliters of the solution containing 0.5 microgram chlorpromazine/ml. The time needed for the measurement is within 10 min. The chlorpromazine radical thus produced is very stable; for example, 95% of the radical was observed after 24 h. The advantage of this method is discussed by comparing with the ordinary spectrophotometry which requires the purification of the sample.

摘要

本文描述了一种用于血液、血清、尿液和组织匀浆中氯丙嗪定量分析的简单且灵敏的方法。向样品中加入高氯酸和2,3-二氯-5,6-二氰基对苯醌所产生的氯丙嗪阳离子自由基，可在室温下通过电子自旋共振（ESR）法进行检测。灵敏度极限为10 ng，即每毫升含0.5微克氯丙嗪的溶液20微升。测量所需时间在10分钟以内。由此产生的氯丙嗪自由基非常稳定；例如，24小时后仍可观察到95%的自由基。通过与需要样品纯化的普通分光光度法进行比较，讨论了该方法的优点。