采用高效液相色谱法分离四环素类药物。
Separation of tetracyclines by high-speed liquid chromatography.
作者信息
Knox J H, Jurand J
出版信息
J Chromatogr. 1975 Jul 2;110(1):103-15. doi: 10.1016/s0021-9673(00)91215-9.
The separation of tetracycline and its four commoninpurities has been studied by high-speed liquid chromatography. A preliminary study of the effectiveness of ion-exchange, adsorption, liquid-liquid partition and reversed-phase ion-pair chromatography indicated that only the last method showed promise. By developing special hydrocarbon-bonded stationary phases a rapid and complete resolution of all five tetracyclines has been obtained within 10 min. Plate heights using a derivatised 18-mum partisil are in the range 0.15-0.3 mm. The method can be used to quantify the impurities in tetracycline at around the 1% level.
采用高效液相色谱法对四环素及其四种常见杂质进行了分离研究。对离子交换、吸附、液 - 液分配和反相离子对色谱法的有效性进行的初步研究表明，只有最后一种方法有前景。通过开发特殊的烃基键合固定相，在10分钟内实现了所有五种四环素的快速、完全分离。使用衍生化的18μm 硅胶柱时，板高在0.15 - 0.3毫米范围内。该方法可用于定量四环素中约1%水平的杂质。
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