Peng J H, Parker J C, Tsai F Y
University of Missouri-Kansas City School of Medicine, Truman Medical Center 64108.
Neurosci Lett. 1991 Sep 2;130(1):37-40. doi: 10.1016/0304-3940(91)90221-e.
The catalytic alpha (alpha) subunit of the human Na,K-ATPase is encoded by multiple genes, as revealed from molecular cloning studies, yet the presence of the alpha 3 isoform has still not been resolved. We investigated the human alpha 3 isoform by Western blotting employing polyclonal anti-rat brain alpha 3 fusion protein, and purified human and rat axolemma Na,K-ATPase preparations. The antibody to rat brain alpha 3 fusion protein cross-reacted with both human and rat antigens, and the stained band superimposed on the alpha 2 band. This result suggests that alpha 3 isoform also exists in human brain.
分子克隆研究显示，人类钠钾ATP酶的催化α亚基由多个基因编码，但α3亚型的存在仍未明确。我们采用多克隆抗大鼠脑α3融合蛋白以及纯化的人和大鼠轴膜钠钾ATP酶制剂，通过蛋白质免疫印迹法对人类α3亚型进行了研究。大鼠脑α3融合蛋白抗体与人及大鼠抗原均发生交叉反应，且染色条带与α2条带重叠。这一结果表明，α3亚型也存在于人类大脑中。
