Radke S, Battmann A, Jatzke S, Eulert J, Jakob F, Schütze N
Orthopaedic Center for Musculoskeletal Research, Molecular Orthopaedics, University of Würzburg, Brettreichstrasse 11, 97074 Würzburg, Germany.
J Orthop Res. 2006 May;24(5):945-52. doi: 10.1002/jor.20097.
Angiogenesis and bone repair are closely linked processes. VEGF, CYR61, and CTGF have been identified as signaling factors that control angiogenesis and could be important in fracture healing. The purpose of this study was to investigate the expression of these signaling factors in osteonecrosis of the femoral head. Twenty-one bone cylinders were retrieved from hips of patients with osteonecrosis of the femoral head at different ARCO stages. Immunohistochemistry for CD34, CYR61, CTGF, and VEGF expression was done on each bone cylinder representing the different regions of osteonecrosis (necrosis, fibrosis, transition zone, and edematous area). VEGF, CYR61, and CTGF were expressed in samples with osteonecrosis. Particularly VEGF and CYR61 were highly expressed in the edematous area. CYR61 was also highly expressed in the transition zone. CTGF was expressed mainly in the area of marrow fibrosis and edema. CYR61, CTGF, and VEGF are expressed to different degrees in the different repair zones of osteonecrosis. Particularly, the high expression of VEGF and CYR61 in the edematous area may represent a consequence of hypoxia and indicate a role of these proteins in the repair processes ongoing in osteonecrosis.
血管生成与骨修复是紧密相连的过程。血管内皮生长因子（VEGF）、富含半胱氨酸的血管生成诱导蛋白61（CYR61）和结缔组织生长因子（CTGF）已被确定为控制血管生成的信号因子，可能在骨折愈合中起重要作用。本研究的目的是调查这些信号因子在股骨头坏死中的表达情况。从处于不同ARCO分期的股骨头坏死患者的髋部获取21个骨圆柱体。对代表股骨头坏死不同区域（坏死区、纤维化区、过渡区和水肿区）的每个骨圆柱体进行CD34、CYR61、CTGF和VEGF表达的免疫组织化学检测。VEGF、CYR61和CTGF在股骨头坏死样本中均有表达。特别是VEGF和CYR61在水肿区高表达。CYR61在过渡区也高表达。CTGF主要在骨髓纤维化和水肿区域表达。CYR61、CTGF和VEGF在股骨头坏死的不同修复区域有不同程度的表达。特别是，VEGF和CYR61在水肿区的高表达可能是缺氧的结果，并表明这些蛋白质在股骨头坏死正在进行的修复过程中发挥作用。
