Tiwari Santosh K, Khan Aleem A, Ibrahim Mohd, Habeeb Mohd Aejaz, Habibullah C M
Centre for Liver Research and Diagnostics, Deccan College of Medical Sciences and Allied Hospitals Kanchanbagh, Hyderabad, Andhra Pradesh, 500 058, India.
World J Gastroenterol. 2006 Apr 14;12(14):2181-6. doi: 10.3748/wjg.v12.i14.2181.
To investigate the presence of Helicobacter species by nested PCR of 16S rRNA genes followed by the presence of Helicobacter pylori (H pylori) 16S rRNA, ureA, cagA genes in bile obtained at endoscopic retrograde cholangio-pancreatography (ERCP) from 60 Indian subjects.
Sixty bile samples were obtained from patients diagnosed with various hepato-biliary diseases and control subjects at ERCP. PCR analysis was carried out using primers for Helicobacter genus 16S rRNA gene and H pylori (16S rRNA, ureA and cagA) genes. Gastric H pylori status was also assessed from biopsies obtained at endoscopy from patients with various hepato-biliary diseases and controls. The control group mainly consisted of subjects with gastric disorders. Sequencing analysis was performed to confirm that PCR products with 16S rRNA and cagA primers were derived from H pylori. RESULTS No Helicobacters were grown in culture from the bile samples. Helicobacter DNA was detected in bile of 96.7% and 6.6% of groups I and II respectively. Ten from group I were positive for 16S rRNA and ureA and 9 were positive for cagA gene. In contrast of the 2 from the control, 1 amplified with 16S rRNA, ureA and cagA primers used. The sequences of the 16S rRNA genes and cagA were 99% similar to Helicobacter pylori.
Helicobacters are associated with the pathogenesis of various hepato-biliary disorders.
通过对16S rRNA基因进行巢式聚合酶链反应（PCR），随后检测幽门螺杆菌（H pylori）的16S rRNA、ureA和cagA基因，来研究60名印度受试者在经内镜逆行胰胆管造影术（ERCP）获取的胆汁中幽门螺杆菌属的存在情况。
在ERCP时从诊断患有各种肝胆疾病的患者和对照受试者中获取60份胆汁样本。使用针对幽门螺杆菌属16S rRNA基因和幽门螺杆菌（16S rRNA、ureA和cagA）基因的引物进行PCR分析。还通过对患有各种肝胆疾病的患者和对照者在内镜检查时获取的活检组织进行评估，来确定胃幽门螺杆菌的状态。对照组主要由患有胃部疾病的受试者组成。进行测序分析以确认16S rRNA和cagA引物的PCR产物源自幽门螺杆菌。结果胆汁样本培养未生长出幽门螺杆菌。I组和II组胆汁中分别有96.7%和6.6%检测到幽门螺杆菌DNA。I组中有10份样本16S rRNA和ureA呈阳性，9份样本cagA基因呈阳性。相比之下，对照组的2份样本中，1份样本使用16S rRNA、ureA和cagA引物扩增呈阳性。16S rRNA基因和cagA的序列与幽门螺杆菌的相似性为99%。
幽门螺杆菌与各种肝胆疾病的发病机制有关。
