蛙皮素诱导猫食管环形平滑肌细胞收缩的信号转导
Signal transduction of bombesin-induced circular smooth muscle cell contraction in cat esophagus.
作者信息
Park Sung-Uk, Shin Chang-Yell, Ryu Jung-Su, La Hyen-O, Park Sun-Young, Song Hyun-Ju, Min Young-Sil, Kim Dong-Seok, Sohn Uy-Dong
机构信息
Department of Pharmacology, College of Pharmacy, Chung Ang University, Seoul 156-756, Republic of Korea.
出版信息
World J Gastroenterol. 2006 Apr 14;12(14):2259-63. doi: 10.3748/wjg.v12.i14.2259.
AIM
To investigate the mechanism of bombesin-induced circular smooth muscle cell contraction in cat esophagus.
METHODS
Specific G protein or phospholipase C involved in cat esophagus contraction was identified, muscle cells were permeabilized with saponin. After permeabilization of muscle cells, the Gi3 antibody inhibited bombesin-induced smooth muscle cell contraction.
RESULTS
Incubation of permeabilized circular muscle cells with PLC-beta3 antibody could inhibit bombesin-induced contraction. H-7, chelerythrine (PKC inhibitor) and genistein (protein tyrosine kinase inhibitor) inhibited bombesin-induced contraction, but DAG kinase inhibitor, R59949, could not inhibit it. To examine which mitogen-activated protein kinase (MAPK) was involved in bombesin-induced contraction, the specific MAPK inhibitors (MEK inhibitor, PD98059 and p38 MAPK inhibitor, SB202190) were used. Preincubation of PD98059 blocked the contraction induced by bombesin in a concentration-dependent manner. However, SB202190 had no effects on contraction.
CONCLUSION
Bombesin-induced circular muscle cell contraction in cat esophagus is madiated via a PKC or a PTK-dependent pathway or p44/p42 MAPK pathway.
目的
研究蛙皮素诱导猫食管环形平滑肌细胞收缩的机制。
方法
鉴定参与猫食管收缩的特定G蛋白或磷脂酶C，用皂角苷使肌肉细胞透化。肌肉细胞透化后，Gi3抗体抑制蛙皮素诱导的平滑肌细胞收缩。
结果
用PLC-β3抗体孵育透化的环形肌细胞可抑制蛙皮素诱导的收缩。H-7、白屈菜红碱（蛋白激酶C抑制剂）和染料木黄酮（蛋白酪氨酸激酶抑制剂）抑制蛙皮素诱导的收缩，但二酰基甘油激酶抑制剂R59949不能抑制。为检测哪种丝裂原活化蛋白激酶（MAPK）参与蛙皮素诱导的收缩，使用了特定的MAPK抑制剂（MEK抑制剂PD98059和p38 MAPK抑制剂SB202190）。PD98059预孵育以浓度依赖方式阻断蛙皮素诱导的收缩。然而，SB202190对收缩无影响。
结论
蛙皮素诱导的猫食管环形肌细胞收缩是通过蛋白激酶C或蛋白酪氨酸激酶依赖性途径或p44/p42 MAPK途径介导的。
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