[杏仁核中央核对下丘脑室旁核引发的心血管反应的作用]

[Roles of the central nucleus of amygdala in the cardiovascular response elicited by the paraventricular nucleus of hypothalamus].

作者信息

Yu Yan-qin, Xia Qiang, Zhang Rong-bao

机构信息

Department of Physiology, College of Medicine, Zhejiang University, Hangzhou 310031, China.

出版信息

Zhejiang Da Xue Xue Bao Yi Xue Ban. 2006 Mar;35(2):172-7. doi: 10.3785/j.issn.1008-9292.2006.02.011.

DOI:10.3785/j.issn.1008-9292.2006.02.011

PMID:16610084

Abstract

OBJECTIVE

To investigate the cardiovascular responses induced by activation of the paraventricular nucleus of hypothalamus (PVN) and the roles of the central nucleus of amygdala (CeA) on this effect.

METHODS

The PVN was activated by microinjection of L-glutamate or electrical stimulation. The CeA was injected with L-glutamate or Kainic acid (KA). The femoral arterial pressure, mean arterial pressure (MAP), electrocardiogram (ECG) and heart rate (HR) of the male SD rats were recorded when the PVN was electrically stimulated.

RESULT

The blood pressure increased when the PVN was activated either by electrical current or by L-glutamate. The blood pressure increased for (10.27+/-1.80)mmHg and the change of heart rate was -10.66 +/- 8.11 beat/min after L-Glu (100 nl) was injected into the ipsilateral CeA. The pressor response of PVN stimulation could still be evoked by electrical stimulation of (13.78 +/- 3.18)mmHg 10 min after kainic acid (100 nl) was injected into the ipsilateral CeA. But this pressor response decreased of 6.57 mmHg compared to that before injection of KA (P <0.05). The locations of the electrode tips and termination of the injector tracts were identified according to the atlas after the recording.

CONCLUSION

Stimulating the PVN elicits pressor response in rats. The CeA mediates partly the pressor response elicited by activation of the PVN.

摘要

目的

研究下丘脑室旁核（PVN）激活所诱导的心血管反应以及杏仁核中央核（CeA）在此效应中的作用。

方法

通过微量注射L-谷氨酸或电刺激激活PVN。向CeA注射L-谷氨酸或海人酸（KA）。在对雄性SD大鼠进行PVN电刺激时，记录其股动脉压、平均动脉压（MAP）、心电图（ECG）和心率（HR）。

结果

当通过电流或L-谷氨酸激活PVN时，血压升高。向同侧CeA注射L-谷氨酸（100 nl）后，血压升高（10.27±1.80）mmHg，心率变化为-10.66±8.11次/分钟。向同侧CeA注射海人酸（100 nl）10分钟后，电刺激仍可诱发PVN刺激的升压反应，幅度为（13.78±3.18）mmHg。但与注射KA前相比，该升压反应降低了6.57 mmHg（P<0.05）。记录结束后，根据图谱确定电极尖端和注射管末端的位置。