Bao Han-ying, Cai Zhen
The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China.
Zhejiang Da Xue Xue Bao Yi Xue Ban. 2006 Mar;35(2):204-8. doi: 10.3785/j.issn.1008-9292.2006.02.017.
To investigate the expression of survivin mRNA in hematological malignancy cells and its correlation with HHT-induced cell apoptosis.
Hematological malignancy cell lines MUTZ-1, K562, Jurkat, RPMI and HL60 were treated with HHT in vitro. Cell apoptosis was observed by flow cytometry (FCM) and the expression of surviving and XIAP mRNA was evaluated with semi-quantitative RT-PCR.
The expression of survivin mRNA on cell lines was negatively correlated to HHT-induced cell apoptotic rate (r=-0.980, P=0.003). There were no significant differences in XIAP expression among these 5 cell lines.
Survivin could be used as a new marker for drug-sensitivity and a new target for treatment of hematological malignancies.
研究存活素（survivin）mRNA在血液系统恶性肿瘤细胞中的表达及其与高三尖杉酯碱（HHT）诱导细胞凋亡的相关性。
采用HHT体外处理血液系统恶性肿瘤细胞株MUTZ-1、K562、Jurkat、RPMI和HL60。应用流式细胞术（FCM）观察细胞凋亡情况，采用半定量逆转录聚合酶链反应（RT-PCR）检测存活素和X连锁凋亡抑制蛋白（XIAP）mRNA的表达。
细胞株中survivin mRNA的表达与HHT诱导的细胞凋亡率呈负相关（r=-0.980，P=0.003）。这5种细胞株之间XIAP表达无显著差异。
Survivin可作为药物敏感性的新指标及血液系统恶性肿瘤治疗的新靶点。
