技术说明：一种基于单克隆抗体的免疫分析法用于测定猪饲料中的莱克多巴胺。

Technical note: a monoclonal antibody-based immunoassay for determination of ractopamine in swine feeds.

作者信息

Wang J P, Zhang S X, Shen J Z

机构信息

Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, China Agricultural University, Beijing 100094, People's Republic of China.

出版信息

J Anim Sci. 2006 May;84(5):1248-51. doi: 10.2527/2006.8451248x.

DOI:10.2527/2006.8451248x

PMID:16612028

Abstract

An ELISA was developed for routine screening of ractopamine in swine feeds. Swine feed samples were extracted and purified, and the aqueous portion of the extract was analyzed for ractopamine using ELISA and confirmed by HPLC. For swine complex feeds containing ractopamine at 2.5 to 40 mg/kg, the average recoveries ranged from 73.1 to 86.5% by ELISA and 81.9 to 98.2% by HPLC. For the swine supplement containing ractopamine at 50 to 400 mg/kg, the average recoveries were 105.5 to 111.4% by ELISA and 89.1 to 92.9% by HPLC. The limit of detection was 0.24 microg/g by ELISA and 0.48 microg/g by HPLC, respectively. Results from the swine complex feeds (P = 0.009) and the supplement (P = 0.005) using ELISA and HPLC were not highly correlated. The ELISA was more sensitive and rapid and less expensive than the HPLC method and could be used for ractopamine screening in swine feeds before confirmation and quantification by other methods, such as HPLC.

摘要

开发了一种酶联免疫吸附测定法（ELISA）用于常规筛查猪饲料中的莱克多巴胺。对猪饲料样品进行提取和纯化，然后使用ELISA分析提取物的水相部分中的莱克多巴胺，并通过高效液相色谱法（HPLC）进行确证。对于莱克多巴胺含量为2.5至40 mg/kg的猪配合饲料，ELISA法的平均回收率为73.1%至86.5%，HPLC法为81.9%至98.2%。对于莱克多巴胺含量为50至400 mg/kg的猪补充饲料，ELISA法的平均回收率为105.5%至111.4%，HPLC法为89.1%至92.9%。ELISA法和HPLC法的检测限分别为0.24 μg/g和0.48 μg/g。使用ELISA法和HPLC法对猪配合饲料（P = 0.009）和补充饲料（P = 0.005）检测的结果相关性不高。ELISA法比HPLC法更灵敏、快速且成本更低，可用于在通过其他方法（如HPLC）进行确证和定量之前对猪饲料中的莱克多巴胺进行筛查。