Chen W R, Okamoto H, Tao Q M
Institute of Hepatology, Beijing Medical University.
Chin Med J (Engl). 1991 Oct;104(10):825-9.
To estimate the homology between Chinese HCV strain, Japanese HCV strain and American HCV strain, we isolated and sequenced 8 clones, representing a 277-long fragment of high diversity. Chinese HCV strain was found to be homologous only in 68.6-72.6% for nucleotide sequence and in 69.6-73.9% for amino acid sequence to American HCV strain, but in 83.4-88.4% for nucleotide sequence and in 78.3-88.0% for amino acid sequence to Japanese main strain. The isolated strain may be the main strain in China, which can be divided into several substrains. This result is believed to be of paramount importance for the development of HCV detection method and vaccination as well as for the study on pathogenesis of HCV infection.
为了评估中国丙型肝炎病毒（HCV）毒株、日本HCV毒株和美国HCV毒株之间的同源性，我们分离并测序了8个克隆，这些克隆代表了一段长度为277且具有高度多样性的片段。结果发现，中国HCV毒株与美国HCV毒株相比，核苷酸序列的同源性仅为68.6 - 72.6%，氨基酸序列的同源性为69.6 - 73.9%；而与日本主要毒株相比，核苷酸序列的同源性为83.4 - 88.4%，氨基酸序列的同源性为78.3 - 88.0%。分离出的毒株可能是中国的主要毒株，并且可以进一步分为几个亚毒株。这一结果对于丙型肝炎病毒检测方法的开发、疫苗接种以及HCV感染发病机制的研究都具有至关重要的意义。
