Lavalette Daniel, Hink Mark A, Tourbez Martine, Tétreau Catherine, Visser Antonie J
Institut Curie-Recherche, Bâtiment 112, Centre Universitaire, 91405, Orsay, France.
Eur Biophys J. 2006 Aug;35(6):517-22. doi: 10.1007/s00249-006-0060-z. Epub 2006 Apr 13.
Translational and rotational diffusion coefficients of proteins in solution strongly deviate from the Stokes-Einstein laws when the ambient viscosity is induced by macromolecular co-solutes rather than by a solvent of negligible size as was assumed by A. Einstein one century ago for deriving the laws of Brownian motion and diffusion. Rotational and translational motions experience different micro viscosities and both become a function of the size ratio of protein and macromolecular co-solute. Possible consequences upon fluorescence spectroscopy observations of diffusing proteins within living cells are discussed.
当溶液中蛋白质的平动和转动扩散系数显著偏离斯托克斯-爱因斯坦定律时，环境粘度是由大分子共溶质引起的，而不是像一个世纪前A.爱因斯坦为推导布朗运动和扩散定律所假设的那样，由尺寸可忽略不计的溶剂引起的。转动和平动运动经历不同的微观粘度，并且都成为蛋白质与大分子共溶质尺寸比的函数。文中讨论了这可能对活细胞内扩散蛋白质的荧光光谱观察结果产生的影响。
