Homaeigohar S S H, Shokrgozar M A, Javadpour J, Khavandi A, Sadi A Yari
National Cell Bank of Iran, Pasteur Institute of Iran, and Department of Materials Science and Engineering, Iran University of Science and Technology, (IUST), Tehran, Iran.
J Biomed Mater Res A. 2006 Jul;78(1):129-38. doi: 10.1002/jbm.a.30691.
Beta-tricalcium phosphate-reinforced high-density polyethylene (beta-TCP/HDPE) is a new biomaterial, which was made to simulate bone composition and study its capacity to act like bony tissues. This material was produced by replacing mineral component and collagen soft tissue of bone with beta-TCP and HDPE, respectively. The biocompatibility of composite samples with different volume fractions of TCP (20, 30, and 40 vol %) and two different particle sizes (80-100 and 120-140 mesh size) was examined in vitro using the osteoblast cell line G-292 by proliferation, alkaline phosphatase (ALP) production, and cell adhesion assays. Cell-material interaction on the surface of the composites was observed by scanning electron microscopy (SEM). The effect of beta-TCP particle size on behavior of the osteoblast cell line was compared between two groups of the composite samples containing smaller and larger reinforcement particle sizes as well as with those of a negative control. In general, results showed that the composite samples containing larger particles supported a higher rate of proliferation and ALP production by osteoblast cells after 3, 7, and 14 days of incubation compared to the composite samples with smaller particle size and control. Furthermore, more cells were attached to the surface of composite samples containing larger particle size when compared to the smaller particle size composites (p<0.05). This number was nearly equal with numbers adhered on negative control [tissue culture polystyrene (TPS)] and significantly higher in comparison with composite control [polyethylene (PE)] (p<0.05). Adhered cells presented a normal morphology by SEM and many of the cells were seen to be undergoing cell division. These findings indicate that beta-TCP/HDPE composites are biocompatible, nontoxic, and in some cases, act to stimulate proliferation of the cells, ALP production, and cell adhesion when compared to the control counterparts. Furthermore, beta-TCP/HDPE samples with larger reinforcement particle size were shown to possess better biological properties.
β-磷酸三钙增强高密度聚乙烯（β-TCP/HDPE）是一种新型生物材料，旨在模拟骨成分并研究其类似骨组织的功能。该材料通过分别用β-TCP和HDPE替代骨的矿物质成分和胶原软组织制成。使用成骨细胞系G-292，通过增殖、碱性磷酸酶（ALP）产生和细胞粘附试验，在体外检测了具有不同体积分数TCP（20%、30%和40%体积）和两种不同粒径（80-100目和120-140目）的复合样品的生物相容性。通过扫描电子显微镜（SEM）观察复合材料表面的细胞-材料相互作用。比较了两组含有较小和较大增强颗粒尺寸的复合样品以及阴性对照样品中β-TCP粒径对成骨细胞系行为的影响。总体而言，结果表明，与较小粒径的复合样品和对照相比，含有较大颗粒的复合样品在孵育3、7和14天后，支持成骨细胞更高的增殖率和ALP产生。此外，与较小粒径的复合材料相比，更多的细胞附着在含有较大粒径的复合样品表面（p<0.05）。这个数字与粘附在阴性对照[组织培养聚苯乙烯（TPS）]上的数字几乎相等，与复合对照[聚乙烯（PE）]相比显著更高（p<0.05）。通过SEM观察，粘附细胞呈现正常形态，并且许多细胞正在进行细胞分裂。这些发现表明，与对照相比，β-TCP/HDPE复合材料具有生物相容性、无毒，并且在某些情况下，可刺激细胞增殖、ALP产生和细胞粘附。此外，具有较大增强颗粒尺寸的β-TCP/HDPE样品显示出更好的生物学性能。
