Guellati M, Ramade F, Le Nguyen D, Ibos F, Bayle J D
Unit of Endocrine Neurobiology (URA 1197 CNRS), University Montpellier II, France.
J Dev Physiol. 1991 Jun;15(6):357-63.
Functional development of the adrenocorticotropic axis was inferred from plasma ACTH and corticosterone levels in intact and embryonically bursectomized (BFX) embryos and chicks from 8 days before to 56 days after hatch. Bursectomy was surgically made at 80 h of incubation and resulted in various alterations in developing adrenocorticotropic axis: ether stress-induced hormonal stimulation could be detected more precociously in BFX (day-6) than in intact embryos; the non stress-responsive period of newly hatched controls did not appear in BFX chicks; BFX young adult chicken exhibited quite smaller responses to stress than controls. In ovo injection of bursin (Lys-His-Gly-NH2) to 6- and 9-days old BFX embryos could restore normal adrenocorticotropic development provided convenient doses of tripeptide were used: administration of 100 fg or 100 pg of bursin was effective to restore normal hormonal levels at all stages studied whereas 100 micrograms was effective at embryonic stages only. The tripeptide Lys-His-Gly-NH2 is suggested as a possible signal from the immune B system directed at the hypothalamo-hypophysial-adrenocortical axis.
通过检测完整胚胎、胚胎期法氏囊切除（BFX）的胚胎以及从孵化前8天到孵化后56天的雏鸡血浆促肾上腺皮质激素（ACTH）和皮质酮水平，推断促肾上腺皮质轴的功能发育情况。在孵化80小时时进行手术切除法氏囊，导致发育中的促肾上腺皮质轴出现各种变化：与完整胚胎相比，BFX胚胎（第6天）能更早检测到乙醚应激诱导的激素刺激；新孵化的对照雏鸡的非应激反应期在BFX雏鸡中未出现；BFX成年雏鸡对应激的反应比对照雏鸡小得多。如果使用合适剂量的三肽，在6日龄和9日龄的BFX胚胎中卵内注射法氏囊素（Lys-His-Gly-NH2）可恢复正常的促肾上腺皮质发育：在所有研究阶段，注射100 fg或100 pg法氏囊素可有效恢复正常激素水平，而100微克仅在胚胎阶段有效。三肽Lys-His-Gly-NH2被认为可能是免疫B系统向下丘脑-垂体-肾上腺皮质轴发出的信号。
