Zhu Yong-Sheng, Guo Xiang, Ge Juan
Second Affiliated Hospital of Nanjing Medical University, Nanjing 210011, China.
Zhongguo Dang Dai Er Ke Za Zhi. 2006 Apr;8(2):97-100.
To investigate the effects of survivin antisense oligonucleotide (ODN) on cell proliferation and apoptosis of HL-60 cells.
Synthetic ODN was completely phosphorothioate-modified. Cationic lipid-mediated antisense ODN was transferred into HL-60 cells. The expression of survivin mRNA and protein was detected by RT-PCR and Western Blot. The incorporation of MTT was used as the measurement of HL-60 proliferation. The cell-cycle and apoptosis were analyzed by flow cytometry.
HL-60 cells spontaneously expressed survivin mRNA and protein. Both mRNA and protein expression of survivin decreased significantly in the antisense ODN transfected cells in comparison to that in the original cells and cells transfected with sense ODN. Survivin antisense ODN significantly inhibited cell proliferation and induced apoptosis in a dose-dependent manner. The cell-cycle in the antisense ODN-transfected cells stopped at the G2/M phase.
Antisense ODN targeting at survivin mRNA can inhibit HL-60 cell proliferation and induce G2/M stop and apoptosis.
探讨生存素反义寡核苷酸（ODN）对HL-60细胞增殖和凋亡的影响。
合成的ODN进行完全硫代磷酸修饰。采用阳离子脂质体介导将反义ODN转入HL-60细胞。通过逆转录聚合酶链反应（RT-PCR）和蛋白质免疫印迹法（Western Blot）检测生存素mRNA和蛋白的表达。采用MTT掺入法检测HL-60细胞的增殖情况。通过流式细胞术分析细胞周期和凋亡情况。
HL-60细胞自发表达生存素mRNA和蛋白。与原代细胞及正义ODN转染细胞相比，反义ODN转染细胞中生存素的mRNA和蛋白表达均显著降低。生存素反义ODN以剂量依赖方式显著抑制细胞增殖并诱导凋亡。反义ODN转染细胞的细胞周期停滞于G2/M期。
靶向生存素mRNA的反义ODN可抑制HL-60细胞增殖，诱导G2/M期停滞及凋亡。
