Han Ying, Qin Jiong, Bu Ding-Fang, Chang Xing-Zhi, Yang Zhi-Xian, Du Jun-Bao
Department of Pediatrics, Peking University First Hospital, Beijing 100034, China.
Zhongguo Dang Dai Er Ke Za Zhi. 2006 Apr;8(2):141-3.
Febrile seizure (FS) is one of the most common seizure types in children. Our previous studies have demonstrated that both gamma-aminobutyric acid B receptor (GABABR) and hydrogen sulfide (H2S) are involved in the pathogenesis of FS. This study was designed to explore the effect of GABABR on H2S/cystathionine-beta-synthase (CBS) system in recurrent FS.
Sixty-four Sprague-Dawley rats aged 21 days were randomly assigned into four groups: Control (37 degrees C water bath exposure), FS, FS+baclofen (GABABR excitomotor), and FS+phaclofen (GABABR inhibitor) groups (n=16 each). FS was induced by warm water bath exposure (45.2 degrees C, once every 2 days, 10 times in total. The plasma level of H2S was detected by the spectrophotometer. The expression of CBS mRNA was examined by in situ hybridization. The expressions of CBS protein was observed by immunohistochemistry.
The plasma level of H2S increased in the FS+baclofen group (427.45 +/- 15.91 micromol/L) but decreased in the FS+phaclofen group (189.72 +/- 21.53 micromol/L) compared with that in the FS group (362.14 +/- 19.71 micromol/L). The expressions of CBS mRNA and protein were up-regulated in the FS+baclofen group but were down-regulated in the FS+phaclofen group compared with those in the FS group.
GABABR modulated the expression of H2S/CBS system in recurrent FS.
热性惊厥（FS）是儿童最常见的惊厥类型之一。我们之前的研究表明，γ-氨基丁酸B受体（GABABR）和硫化氢（H2S）均参与FS的发病机制。本研究旨在探讨GABABR对复发性FS中H2S/胱硫醚-β-合酶（CBS）系统的影响。
将64只21日龄的Sprague-Dawley大鼠随机分为四组：对照组（37℃水浴暴露）、FS组、FS+巴氯芬组（GABABR激动剂）和FS+法氯芬组（GABABR抑制剂）（每组n = 16）。通过温水浴暴露诱导FS（45.2℃，每2天1次，共10次）。用分光光度计检测血浆H2S水平。通过原位杂交检测CBS mRNA的表达。通过免疫组织化学观察CBS蛋白的表达。
与FS组（362.14±19.71μmol/L）相比，FS+巴氯芬组血浆H2S水平升高（427.45±15.91μmol/L），而FS+法氯芬组降低（189.72±21.53μmol/L）。与FS组相比，FS+巴氯芬组CBS mRNA和蛋白的表达上调，而FS+法氯芬组下调。
GABABR调节复发性FS中H2S/CBS系统的表达。
