von Maltzahn Julia, Wulf Volker, Willecke Klaus
Institut für Genetik, Abteilung Molekulargenetik, Universität Bonn, Bonn, Germany.
Cell Commun Adhes. 2006 Jan-Apr;13(1-2):55-60. doi: 10.1080/15419060600631508.
Connexin39 (Cx39) and connexin43 (Cx43) are known to be expressed during development of skeletal muscles. Here we have compared the expression pattern of both connexins during differentiation of established C(2)C(12) mouse myoblasts and in the mouse embryo. Cx43 is highly abundant in undifferentiated myoblasts, but no Cx39 protein was detected in these cells. Upon differentiation into myotubes, Cx39 expression increased. The consecutive expression of these connexins was also observed in the mouse embryo. Cx39 and Cx43 were found in different plaques in accordance with the notion that Cx43 is exclusively expressed in myoblasts and Cx39 in myotubes. Thus, differentiating C(2)C(12) cells in culture can serve to study the involvement of gap junctions in myogenesis, since expression of corresponding Cx39 and Cx43 proteins appears to be very similar as in the mouse embryo.
已知连接蛋白39（Cx39）和连接蛋白43（Cx43）在骨骼肌发育过程中表达。在此，我们比较了这两种连接蛋白在已建立的C(2)C(12)小鼠成肌细胞分化过程以及小鼠胚胎中的表达模式。Cx43在未分化的成肌细胞中高度丰富，但在这些细胞中未检测到Cx39蛋白。在分化为肌管后，Cx39的表达增加。在小鼠胚胎中也观察到了这些连接蛋白的连续表达。根据Cx43仅在成肌细胞中表达而Cx39在肌管中表达的观点，发现Cx39和Cx43存在于不同的斑块中。因此，培养中分化的C(2)C(12)细胞可用于研究间隙连接在肌生成中的作用，因为相应的Cx39和Cx43蛋白的表达似乎与小鼠胚胎中的非常相似。
