Balaban Basak, Urman Bulent, Yakin Kayhan, Isiklar Aycan
Assisted Reproduction Unit, American Hospital of Istanbul, Istanbul, Turkey.
Hum Reprod. 2006 Aug;21(8):2136-40. doi: 10.1093/humrep/del097. Epub 2006 Apr 13.
Cryopreservation of embryos may lead to zona hardening that may compromise in vivo hatching and implantation following thawing and transfer. Assisted hatching (AH) has been advocated as a means of assisting the natural hatching process and enhancing implantation.
The aim of this study was to assess in a prospective randomized manner the effect of laser-assisted hatching (LAH) on implantation as well as clinical and multiple pregnancy rates (the primary outcome) after the transfer of frozen-thawed embryos. All embryos were thawed the day before transfer, and LAH was performed the next day on embryos that cleaved. Control group consisted of embryos that were transferred without AH.
The performance of LAH significantly increased implantation (9.9 versus 20.1%, P < 0.01), clinical pregnancy (27.3 versus 40.9, P < 0.05) and multiple pregnancy rates (16 versus 40.3%, P < 0.07). In the LAH group, significantly more excess embryos that were left in culture hatched in vitro.
LAH improves the outcome of frozen-thawed embryo transfer when performed before transfer on embryos that were allowed to cleave.
胚胎冷冻保存可能导致透明带硬化，这可能会损害解冻和移植后胚胎在体内的孵化和着床。辅助孵化（AH）已被提倡作为辅助自然孵化过程和提高着床率的一种方法。
本研究的目的是以前瞻性随机方式评估激光辅助孵化（LAH）对冷冻解冻胚胎移植后的着床率以及临床妊娠率和多胎妊娠率（主要结局）的影响。所有胚胎在移植前一天解冻，第二天对已分裂的胚胎进行LAH。对照组由未进行辅助孵化而移植的胚胎组成。
LAH显著提高了着床率（9.9%对20.1%，P<0.01）、临床妊娠率（27.3对40.9，P<0.05）和多胎妊娠率（16%对40.3%，P<0.07）。在LAH组中，留在培养中的多余胚胎在体外孵化的明显更多。
在移植前对已分裂的胚胎进行LAH可改善冷冻解冻胚胎移植的结局。
