Nälsén Cecilia, Vessby Bengt, Berglund Lars, Uusitupa Matti, Hermansen Kjeld, Riccardi Gabrielle, Rivellese Angela, Storlien Len, Erkkilä Arja, Ylä-Herttuala Seppo, Tapsell Linda, Basu Samar
The KANWU Study Group at Clinical Nutrition and Metabolism, Department of Public Health and Caring Sciences, Uppsala University, Sweden.
J Nutr. 2006 May;136(5):1222-8. doi: 10.1093/jn/136.5.1222.
(n-3) Fatty acids are unsaturated and are therefore easily subject to oxidization; however, they have several beneficial health effects, which include protection against cardiovascular diseases. The aim of this study was to investigate whether (n-3) fatty acids, with a controlled fat quality in the background diet, affect nonenzymatic and enzymatic lipid peroxidation and antioxidant status in humans. A total of 162 men and women in a multicenter study (The KANWU study) were randomly assigned to a diet containing a high proportion of saturated fatty acids or monounsaturated fatty acids (MUFA) for 3 mo. Within each diet group, there was a second random assignment to supplementation with fish-oil capsules [3.6 g (n-3) fatty acids/d] or placebo. Biomarkers of nonenzymatic and enzymatic lipid peroxidation in vivo were determined by measuring 8-iso-prostaglandin F(2alpha) (8-iso-PGF(2alpha)) and prostaglandin F(2alpha) (PGF(2alpha)) concentrations in plasma at baseline and after 3 mo. Antioxidant status was determined by measuring plasma antioxidant capacity with an enhanced chemiluminescence assay. The plasma 8-iso-PGF(2alpha) concentration was significantly decreased after 3 mo of supplementation with (n-3) fatty acids (P = 0.015), whereas the PGF(2alpha) concentration was not affected. The antioxidant status was not affected by supplementation of (n-3) fatty acids, but was improved by the background diet with a high proportion of MUFA. We conclude that supplementation with (n-3) fatty acids decreases nonenzymatic free radical-catalyzed isoprostane formation, but does not affect cyclooxygenase-mediated prostaglandin formation.
(n-3)脂肪酸是不饱和脂肪酸，因此容易被氧化；然而，它们对健康有多种有益影响，包括预防心血管疾病。本研究的目的是调查在背景饮食脂肪质量得到控制的情况下，(n-3)脂肪酸是否会影响人体的非酶促和酶促脂质过氧化以及抗氧化状态。在一项多中心研究（KANWU研究）中，共有162名男性和女性被随机分配到含有高比例饱和脂肪酸或单不饱和脂肪酸（MUFA）的饮食组中，为期3个月。在每个饮食组内，再次随机分配补充鱼油胶囊[3.6克(n-3)脂肪酸/天]或安慰剂。通过测量基线时和3个月后的血浆中8-异前列腺素F(2α)（8-iso-PGF(2α)）和前列腺素F(2α)（PGF(2α)）浓度来测定体内非酶促和酶促脂质过氧化的生物标志物。通过增强化学发光法测量血浆抗氧化能力来确定抗氧化状态。补充(n-3)脂肪酸3个月后，血浆8-iso-PGF(2α)浓度显著降低（P = 0.015），而PGF(2α)浓度未受影响。(n-3)脂肪酸补充剂未影响抗氧化状态，但高比例MUFA的背景饮食改善了抗氧化状态。我们得出结论，补充(n-3)脂肪酸可减少非酶促自由基催化的异前列腺素形成，但不影响环氧化酶介导的前列腺素形成。
