Departament of Nutrition, Health Sciences Center, Federal University of State of Rio Grande do Norte, Avenida Senador Salgado Filho, n° 3000, Natal, RN 59078-970 Brazil.
2Laboratório de Pesquisa do Exercício, Escola de Educação Física, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS 90690-200 Brazil.
J Int Soc Sports Nutr. 2018 Apr 23;15:18. doi: 10.1186/s12970-018-0222-2. eCollection 2018.
The relationship between diabetes and oxidative stress has been previously reported. Exercise represents a useful non-pharmacological strategy for the treatment in type 2 diabetic (T2DM) patients, but high intensity exercise can induce a transient inflammatory state and increase oxidative stress. Nutritional strategies that may contribute to the reduction of oxidative stress induced by acute exercise are necessary. The aim of this study was to examine if n-3 PUFA supplementation intervention can attenuate the inflammatory response and oxidative stress associated with high intensity exercise in this population. As a primary outcome, lipoperoxidation measurements (TBARS and F2-isoprostanes) were selected.
Thirty T2DM patients, without chronic complications, were randomly allocated into two groups: placebo (gelatin capsules) or n-3 PUFA (capsules containing 180 mg of eicosapentaenoic acid and 120 mg of docosahexaenoic acid). Blood samples were collected fasting before and after 8 weeks supplementation. In the beginning and at the end of protocol, an acute exercise was performed (treadmill), and new blood samples were collected before and immediately after the exercise for measurements of oxidative stress and high-sensitivity C-reactive protein (hs-CRP).
After the supplementation period, a decrease in triglycerides levels was observed only in n-3 PUFA supplementation group (mean difference and 95% CI of 0.002 (0.000-0.004), = 0.005). Supplementation also significantly reduced TRAP levels after exercise (mean difference and 95% CI to 9641 (- 20,068-39,351) for - 33,884 (- 56,976 - -10,793), = 0.004, Cohen's effect size = 1.12), but no significant difference was observed in n-3 PUFA supplementation group in lipoperoxidation parameters as TBARS (mean difference and 95% CI to - 3.8 (- 10-2.4) for - 2.9 (- 1.6-7.4) or F2-isoprostanes (mean difference and 95% CI -0.05 (- 0.19-0.10) for - 0.02 (- 0.19-0.16), > 0.05 for both.
PUFA n-3 supplementation reduced triglycerides as well as TRAP levels after exercise, without a significant effect on inflammatory and oxidative stress markers.This study is registered at ClinicalTrials.gov with the registration number of NCT03182712.
糖尿病与氧化应激之间的关系已被先前报道过。运动是治疗 2 型糖尿病（T2DM）患者的一种有用的非药物策略，但高强度运动可诱导短暂的炎症状态并增加氧化应激。需要寻找可能有助于减少急性运动引起的氧化应激的营养策略。本研究旨在研究 n-3 PUFA 补充干预是否可以减轻该人群高强度运动引起的炎症反应和氧化应激。作为主要结果，选择了脂质过氧化测量（TBARS 和 F2-异前列腺素）。
30 名无慢性并发症的 T2DM 患者被随机分配到两组：安慰剂（明胶胶囊）或 n-3 PUFA（胶囊含有 180mg 二十碳五烯酸和 120mg 二十二碳六烯酸）。在补充 8 周前和后空腹采集血液样本。在方案开始和结束时，进行急性运动（跑步机），并在运动前后立即采集新的血液样本，以测量氧化应激和高敏 C 反应蛋白（hs-CRP）。
补充期后，仅在 n-3 PUFA 补充组观察到甘油三酯水平降低（平均差异和 95%CI 为 0.002（0.000-0.004），= 0.005）。补充还显著降低了运动后的 TRAP 水平（平均差异和 95%CI 为 9641（-20，068-39，351）到-33，884（-56，976- -10，793），= 0.004，Cohen's 效应大小= 1.12），但 n-3 PUFA 补充组的脂质过氧化参数 TBARS（平均差异和 95%CI 为-3.8（-10-2.4）到-2.9（-1.6-7.4）或 F2-异前列腺素（平均差异和 95%CI -0.05（-0.19-0.10）到-0.02（-0.19-0.16），均无显著差异，>0.05）。
PUFA n-3 补充剂可降低运动后的甘油三酯和 TRAP 水平，而对炎症和氧化应激标志物无显著影响。本研究在 ClinicalTrials.gov 注册，注册号为 NCT03182712。
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