Lehmann Ch, Bac V H, Pavlovic D, Lustig M, Maier S, Feyerherd F, Usichenko T-I, Meissner K, Haase H, Jünger M, Wendt M, Heidecke C-D, Gründling M
Klinik für Anästhesiologie und Intensivmedizin, Ernst-Moritz-Arndt-Universität Greifswald, Germany.
Clin Hemorheol Microcirc. 2006;34(3):427-38.
To explore the effects of metronidazole (Me) on intestinal microcirculation in septic rats, intravital microscopy (IVM) following 16 hours of colon ascendens stent peritonitis (CASP model) was used. Four groups of animals were studied: control group (sham operation) and CASP group, each with and without Me treatment (10 mg/kg i.v.). In order to investigate the substance-specific effects of Me independently of the antibacterial effects within a pathologically altered microcirculation, a second experimental series with lipopolysaccharide challenge (LPS model) was carried out. The LPS model consisted of the four groups (control animals and LPS animals (15 mg/kg i.v. LPS from E. coli) with and without Me). IVM in the LPS experiments was performed following a two hour observation period. Me treated CASP or LPS animals, as compared with untreated, demonstrated significant improvement of functional capillary density (FCD) of the intestinal wall. The increase in the number of leukocytes firmly adhered to the endothelium (leukocyte sticking) in the untreated CASP or LPS animals within the V1 venules of the intestinal submucosal layer, was significantly reduced in the Me treated animals. In conclusion, Me exerts beneficial anti-bacterial and anti-inflammatory effects within the septic microcirculation.
为探讨甲硝唑（Me）对脓毒症大鼠肠道微循环的影响，采用升结肠支架诱导腹膜炎16小时后的活体显微镜检查（IVM）（CASP模型）。研究了四组动物：对照组（假手术）和CASP组，每组又分为接受和未接受Me治疗（静脉注射10 mg/kg）的亚组。为了在病理改变的微循环中独立于抗菌作用研究Me的物质特异性作用，进行了第二个脂多糖激发实验系列（LPS模型）。LPS模型由四组组成（对照组动物和LPS组动物（静脉注射15 mg/kg大肠杆菌来源的LPS），每组又分为接受和未接受Me治疗的亚组）。LPS实验中的IVM在两小时观察期后进行。与未治疗的动物相比，接受Me治疗的CASP或LPS动物肠壁的功能性毛细血管密度（FCD）有显著改善。在未治疗的CASP或LPS动物的肠黏膜下层V1小静脉中，牢固黏附在内皮上的白细胞数量（白细胞黏附）增加，而接受Me治疗的动物中这一现象显著减少。总之，Me在脓毒症微循环中发挥有益的抗菌和抗炎作用。
