Yu Ju-Hyun, Klibanov Alexander M
Department of Chemistry and Division of Biological Engineering, Massachusetts Institute of Technology, Cambridge, 02139, USA.
Biotechnol Lett. 2006 Apr;28(8):555-8. doi: 10.1007/s10529-006-0018-3.
The impact of co-lyophilizing horseradish peroxidase (HRP) with numerous amino acids and other chiral excipients on the enzyme's subsequent stereoselectivity [E(S/R)] in the sulfoxidation of thioanisole in 2-propanol was systematically investigated. While many improved the stereoselectivity of (and significantly activated) HRP, the greatest effect was observed with D-proline which enhanced the E(S/R) value by over an order of magnitude from synthetically meaningless to useful.
系统研究了辣根过氧化物酶（HRP）与多种氨基酸及其他手性辅料共冻干对其随后在2-丙醇中硫代苯甲醚亚砜化反应中的立体选择性[E(S/R)]的影响。虽然许多物质提高了HRP的立体选择性（并显著激活了它），但D-脯氨酸的效果最为显著，它将E(S/R)值从无合成意义提高到有用水平，提高了一个数量级以上。
