人CD34+造血干细胞和祖细胞培养前后的差异基因表达。

Differential gene expression of human CD34+ hematopoietic stem and progenitor cells before and after culture.

作者信息

Li Qunliang, Cai Haibo, Liu Qiwei, Tan Wen-Song

机构信息

The State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 200237, Shanghai, China.

出版信息

Biotechnol Lett. 2006 Mar;28(6):389-94. doi: 10.1007/s10529-005-6064-4.

DOI:10.1007/s10529-005-6064-4

PMID:16614904

Abstract

Ex vivo expanded CD34+ hematopoietic stem and progenitor cells (HSPCs) have compromised homing and engraftment capacities. To investigate underlying mechanisms for functional changes of expanded HSPCs, we compared gene expression profiling of cultured and fresh CD34+ cells derived from cord blood using SMART-PCR and cDNA array: 20 genes were up-regulated while 25 genes were down-regulated in cultured CD34+ HSPCs. These differentially expressed genes are involved primarily in proliferation, differentiation, apoptosis, and homing.

摘要

体外扩增的CD34+造血干细胞和祖细胞（HSPCs）的归巢和植入能力受损。为了研究扩增的HSPCs功能变化的潜在机制，我们使用SMART-PCR和cDNA阵列比较了来自脐带血的培养CD34+细胞和新鲜CD34+细胞的基因表达谱：在培养的CD34+ HSPCs中，20个基因上调，25个基因下调。这些差异表达的基因主要参与增殖、分化、凋亡和归巢。