盐生杜氏藻（6-4）光裂合酶编码基因的克隆与序列分析。

Cloning and sequence analysis of the gene encoding (6-4)photolyase from Dunaliella salina.

作者信息

Yi Yi, Yi Cao, Qian Li, Min Liu, Long Cheng, Linhan Bai, Zhirong Yang, Dairong Qiao

机构信息

Key Laboratory of Bio-resources and Eco-environment, Sichuan University, Ministry of Education, 610064, Chengdu, Sichuan Province, P. R. China.

出版信息

Biotechnol Lett. 2006 Mar;28(5):309-14. doi: 10.1007/s10529-005-5716-8.

DOI:10.1007/s10529-005-5716-8

PMID:16614917

Abstract

DNA photolyase can repair UV-induced DNA damage in a light-dependent manner. A cDNA of (6-4)photolyase from Dunaliella salina (GenBank accession number: AY845324) was cloned, sequenced and its amino acid sequence was deduced. The derived amino acid sequence showed high homology with other (6-4)photolyases and a predicted 3D model was constructed by homology modeling.

摘要

DNA光解酶能够以光依赖的方式修复紫外线诱导的DNA损伤。克隆并测序了盐生杜氏藻（GenBank登录号：AY845324）的(6-4)光解酶的cDNA，并推导了其氨基酸序列。推导的氨基酸序列与其他(6-4)光解酶具有高度同源性，并通过同源建模构建了预测的三维模型。