人造血干/祖细胞体外扩增的动力学分析

Kinetic analysis of the ex vivo expansion of human hematopoietic stem/progenitor cells.

作者信息

Gonçalves Raquel, da Silva Cláudia Lobato, Ferreira Bruno S, Lemos Maria Amélia N D A, Lemos Francisco, Almeida-Porada Graça, Cabral Joaquim M S

机构信息

Centro de Engenharia Biológica e Química, Instituto Superior Técnico, Av. Rovisco Pais, 1049-001, Lisboa, Portugal.

出版信息

Biotechnol Lett. 2006 Mar;28(5):335-40. doi: 10.1007/s10529-005-5932-2.

DOI:10.1007/s10529-005-5932-2

PMID:16614921

Abstract

The total cell expansion of human umbilical cord blood (CB) and adult bone marrow (BM) CD34+-enriched cells cultured in supplemented serum-free media, either over irradiated human feeder layers or in stroma-free systems, were characterized by a simple kinetic model using only two parameters: the specific cell expansion rate, mu, and the death rate constant, k(k). Both CB and BM cells can expand at approximately the same rate (0.21 day(-1)) in this culture system however, cell death depends on the presence of stroma and the environment in which the cells are cultured.

摘要

在添加了无血清培养基中培养的人脐带血（CB）和成人骨髓（BM）富含CD34 +的细胞，无论是在照射过的人饲养层上还是在无基质系统中，其总细胞扩增情况都通过一个仅使用两个参数的简单动力学模型来表征：比细胞扩增率μ和死亡率常数k(k)。在该培养系统中，CB和BM细胞都能以大致相同的速率（0.21天-1）扩增，然而，细胞死亡取决于基质的存在以及细胞培养的环境。