Björklöf Katarina, Färdig Michael, Jørgensen Kirsten S
Finnish Environment Institute, Research Department, Hakuninmaantie 6, FI-00430, Helsinki, Finland.
Biotechnol Lett. 2006 Mar;28(5):341-9. doi: 10.1007/s10529-005-5933-1.
Agrobacterium transformation was used in the production of genetically modified plants from oilseed rape (Brassica napus) and tobacco (Nicotiana tabacum). After inoculation stop with the antibiotic timentin, a subsequent one-week treatment eliminated the vector bacterium from the oilseed rape plate explant cultures. From the tobacco, however, we recorded vector-derived signals one week after potting the regenerants in the greenhouse and still 10 weeks later. Genetically modified plants produced through Agrobacterium-transformation therefore cannot be guaranteed to be completely free of unintended vector sequences after antibiotic treatment.
农杆菌转化法被用于从油菜（甘蓝型油菜）和烟草（烟草）中生产转基因植物。在用抗生素替门汀接种停止后，随后一周的处理从油菜平板外植体培养物中消除了载体细菌。然而，对于烟草，在将再生植株移栽到温室一周后以及10周后，我们仍记录到了源自载体的信号。因此，经农杆菌转化产生的转基因植物在抗生素处理后不能保证完全不含意外的载体序列。
