Ganske Franka, Bornscheuer Uwe T
Department of Technical Chemistry and Biotechnology, Institute of Chemistry and Biochemistry, Greifswald University, Greifswald, Germany.
Biotechnol Lett. 2006 Apr;28(7):465-9. doi: 10.1007/s10529-006-0006-7.
The influence of the two most commonly used ionic liquids (1-butyl-3-methyl imidazolium tetrafluoroborate, [BMIM][BF(4)], 1-butyl-3-methyl imidazolium hexafluorophosphate, [BMIM][PF(6)]) and three selected organic solvents (dimethylsulfoxide, ethanol, methanol) on the growth of Escherichia coli, Pichia pastoris and Bacillus cereus was investigated. [BMIM][BF(4)] was toxic at 1% (v/v) on all three microorganisms. The minimal inhibitory concentration (MIC) of [BMIM][BF(4)] on E. coli growth was between 0.7 and 1% (v/v). In contrast, [BMIM][PF(6)] was less toxic for P. pastoris and B. cereus, whereas E. coli was not able to tolerate [BMIM][PF(6)] (MIC value: 0.3-0.7% v/v). Growth of P. pastoris was unaffected by [BMIM][PF(6)] at 10% (v/v). Similar results were found for dimethylsulfoxide. Thus, ionic liquids (ILs) can have substantial inhibitory effects on the growth of microorganisms, which should be taken into account for environmental reasons as well as for the use of ILs as co-solvents in biotransformations.
研究了两种最常用的离子液体（1-丁基-3-甲基咪唑四氟硼酸盐，[BMIM][BF₄]，1-丁基-3-甲基咪唑六氟磷酸盐，[BMIM][PF₆]）和三种选定的有机溶剂（二甲基亚砜、乙醇、甲醇）对大肠杆菌、毕赤酵母和蜡样芽孢杆菌生长的影响。[BMIM][BF₄]在1%（v/v）时对所有三种微生物均有毒性。[BMIM][BF₄]对大肠杆菌生长的最低抑菌浓度（MIC）在0.7%至1%（v/v）之间。相比之下，[BMIM][PF₆]对毕赤酵母和蜡样芽孢杆菌的毒性较小，而大肠杆菌无法耐受[BMIM][PF₆]（MIC值：0.3 - 0.7% v/v）。在10%（v/v）时，[BMIM][PF₆]对毕赤酵母的生长没有影响。二甲基亚砜也得到了类似的结果。因此，离子液体（ILs）对微生物的生长可能具有显著的抑制作用，出于环境原因以及在生物转化中使用离子液体作为助溶剂的考虑，这一点应予以重视。
